glucose 6- phosphate dehydrogenase from streptomyces aureofaciens was purified andinactivated by pyridoxal 5′-phosphate (plp). the inactivation was a pseudo-first order and time-dependentreaction. complete inactivation was achieved at 0.2mm plp within 16 minutes. the type of inhibition wascompetitive with respect to glucose 6- phosphate. spectral characteristics of plp-enzyme complexcorresponde...

interaction of glucose 6-phosphate dehydrogenase from s. aureofaciens with nad+, nadp+and glucose 6-phosphate were investigated using different fluorescent probes. binding of nad+, nadp+and s-nadph to the native enzyme quenched intrinsic protein fluorescence by 100%, 10% and 21%,respectively, from which kd values of nad+ (6.5 mm), nadp+ (92.0 μm) and s-nadph (122.0 μm)were calculated. binding o...

The soil bacterium Bacillus subtilis can use sugars or organic acids as sources of carbon and energy. These nutrients are metabolized by glycolysis, the pentose phosphate pathway, and the Krebs citric acid cycle. While the response of B. subtilis to the availability of sugars is well understood, much less is known about the changes in metabolism if organic acids feeding into the Krebs cycle are...

The normal human red blood cell, when withdrawn from the circulation, contains a substantial pool of water-soluble organic phosphate compounds, most of which are metabolic intermediates whose concentrations are maintained at constant levels by the glucose metabolism of the cell (1). If blood is stored under blood bank conditions in acid citrate dextrose (ACD) preservative, the organic phosphate...

This study was aimed at developing value-added low sodium chloride (NaCl), phosphate-free restructured patties using minced channel catfish (Ictalurus punctatus) belly flap meat. The effect of microbial transglutaminase (MTGase) and nonmeat proteins (isolated soy protein, ISP, and whey protein concentrate, WPC; 1.7%, respectively) alone and in combination were evaluated to improve cooking yield...

Recently, consumers have become more concerned with foods contain natural ingredients in terms of health and economic. Hence, in this study, the effects of concentrate of smoked and sun dried raisin were investigated at 3 levels (1, 3 and 5%) as a natural humectant to improve the quality and shelf life of flat bread. Results showed that addition of smoked and sun dried raisin improved quality a...

In the frame of increasing need nutrient recovery and recycling objective was to fractionate concentrate nutrients from nitrified, ultra-filtered ultraviolet C treated UASB effluent with anion selectrodialysis (aSED). Under these conditions it possible keep system in operation for 14 days or even 3 weeks intermittent cleaning place every week. When membranes developed sulphuric acid production ...

in this research work, the zinc extraction was investigated, using bioleaching process from a low grade zinc concentrate which was produced from the accumulated flotation tailings. zinc content was initially upgraded to 11.97% by flotation process. bioleaching experiments were designed and carried out by a mixed culture of acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans, leptospir...

utilizing natural clay in the traditional production of the grape concentrates in the villages cause the input of large amounts of some metals (solutes) in the product which will be harmful for the consumers. to eliminate the natural clay from this procedure in non-industrial scale, bentonite was selected as an aid-clarifier and examined in treatments under these conditions: two levels of bento...