INTRODUCTION The objective of this study was to compare the clinical usefulness of the new anti-double-stranded DNA nucleosome-complexed enzyme-linked immunosorbent assay (Anti-dsDNA-NcX ELISA), which is based on dsDNA-loaded nucleosomes as antigens, with established test systems based on dsDNA or nucleosomes alone for systemic lupus erythematosus (SLE) diagnostics and determination of disease ...

Aim: The aim of the study was to determine burden and epidemiology rubella infection in Lesotho before introduction a rubella-containing vaccine. It also assess performance measles case-based surveillance system Lesotho.
 Study Design: Retrospective cross-sectional descriptive study.
 Place Duration Study: done from February March 2019 based on 2012 2016 data.
 Methods: There rev...

In systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM), auto-antibodies are used in daily practice as potent biomarkers of clinical phenotypes. This study aimed at estimating the prevalence myositis-specific (MSA) myositis-associated (MAA) a well-characterised SSc patients cohort using two different immunoblot assays, studying their associations.In this cross-sectional study, ...

OBJECTIVE To identify the hematological manifestations and its association with serum ferritin levels in SLE patients from Western India. METHODS Ninety clinically diagnosed SLE patients fulfilling ACR criteria were included. Disease activity was assessed at the time of evaluation using Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Sera were tested for serum ferritin levels by...

BACKGROUND IgA- and IgG-antibodies against deamidated gliadin peptides (DGP) specifically bind the disease-inducing antigen and might be superior to transglutaminase type 2 (TG2) IgA in monitoring patients on a gluten-free diet (GFD). The aim of this study was to compare the performance of DGP-IgG and DGP-IgA with TG2-IgA of four manufacturers in pediatric celiac patients at diagnosis and durin...

Autoantibodies to the Sm antigens are specifically found in 5 to 30% of patients with systemic lupus erythematosus (SLE) depending on the detection system and the patient group. Several immunoassays designed for research and diagnostic laboratory use have been developed. The autoantigens employed in these tests include purified native proteins, recombinant polypeptides, and synthetic peptides. ...

OBJECTIVE The diagnostic accuracy of cerebrospinal fluid (CSF) biomarkers for Alzheimer's disease (AD) must be improved before widespread clinical use. This study aimed to determine whether CSF Aβ42/Aβ40 and Aβ42/Aβ38 ratios are better diagnostic biomarkers of AD during both predementia and dementia stages in comparison to CSF Aβ42 alone. METHODS The study comprised three different cohorts (n...

Dermatomyositis (DM) is a major clinical subset of autoimmune myositis (AIM). The characteristic DM rash (Gottron papules, heliotrope rash) and perifascicular atrophy at skeletal muscle biopsy are regarded as specific features for this diagnosis. However, new concepts are challenging the current definition of DM. A modified Bohan and Peter classification of AIM was proposed in which the core co...

Background: Since the risk of infection and poor outcomes seem also largely dependent on efficient anti-SARS-CoV-2 IgA response, assessing response this antibody class after vaccination is pivotal. Methods: We analyzed serum levels total anti-spike receptor binding domain (RBD) Ig, (Elecsys Anti-SARS-CoV-2 S immunoassay Roche Cobas 6000; Diagnostics), RBD IgG (ACCESS SARS-CoV-2 II Access 2; ...

been reported about food allergens. We reviewed data from our laboratory and outpatient records of patients consulted to estimate the magnitude of the disease in our population. Methods: Patients attending the only specialist allergy diagnostic facility in the country (Asthma, Allergy and Immune Dysfunction Clinic) were offered semi-quantitative allergen specific IgE antibody determination as p...