Eleven fluorescein isothiocyanate-conjugated (FITC) lectins, each with distinct carbohydratebinding properties, were used to assess cell surface glycoconjugates of embryonic and early postnatal cerebellar cells in vitro. Fluorescence staining of embryonic day 13 (E13) cerebellar cells with FITC Ricinus communis agglutinin diminished markedly between 24 and 72 hr in vitro. No staining of postnat...

Figure S1. The stimulus profile visualized using FITC-BSA shows similar pattern to that of fluorescein, although FITC-BSA pattern are more dispersed compared to that of fluorescein. Profiles were generated by alternatively delivering FITC-BSA solution and PBS at 50 mHz driven by (A) 1psi, (B) 2psi, (C) 3psi and (D) 4psi. Heatmaps of spatial (Y axis) and temporal (X axis) show fluorescent intens...

Abstract Teucrium polium has been used in traditional medicine around the world for centuries treatment of various conditions and diseases. Many studies have confirmed pharmacological effects its extracts, although immunomodulatory effect not investigated. Therefore, aim our study was to examine methanolic extract T. (TPE) on peripheral blood mononuclear cells (PBMCs) derived from healthy donor...

In the interest of classifying cases of male factor infertility, we have paid particular attention to the sugar ligand binding properties of the human sperm surface and the functional capacity of the acrosome for exocytosis--key parameters for assessing sperm fertilizing ability. Zona recognition and binding involve the interactions of sperm surface mannose receptors (lectins) with mannose liga...

The acrosome reaction (AR), an essential step for achieving mammalian fertilization, was recently introduced as a means of clinical evaluation of male fertility. However, most of the available techniques for acrosomal status assessment (except those employing electron microscopy) do not define whether the measurements represent partial or complete AR. We, therefore, performed a crossover invest...

Inactivity in nematodes is often correlated with survival of adverse environments. The non-feeding second-stage juvenile (J2) of Heterodera glycines must survive in a soil environment that may contain numerous toxins. In this report, we show that quiescent J2 of H. glycines survived higher concentrations of both ethanol and the plant-derived compound, allyl isothiocyanate, compared with activel...

The mechanism underlying the handling of protein and peptide drugs such as insulin in alveolar epithelial cells is still unclear. We therefore examined fluorescein isothiocyanate-labeled (FITC)-insulin uptake in rat primary cultured alveolar type II epithelial cells and in transdifferentiated type I-like cells. FITC-insulin uptake in these cells was much higher than those of FITC-immunoglobulin...

Unlike skeletal muscle sarcoplasmic reticulum, canine cardiac sarcoplasmic reticulum hydrolyzes GTP in ways that are similar and different from ATP hydrolysis. Also, ATP and ATP analogues inhibit GTPase activity noncompetitively with a Ki compatible with the high affinity ATP-binding site (c.f. Tate, C.A., Bick, R.J., Blaylock, S., Youker, K., Scherer, N.M., and Entman, M.L. (1989) J. Biol. Che...

BACKGROUND The gastrointestinal tract is the first target for the potentially harmful effects of mycotoxins after intake of mycotoxin contaminated food or feed. With deoxynivalenol (DON), T-2 toxin (T-2), fumonisin B1 (FB1) and zearalenone (ZEA) being important Fusarium toxins in the northern hemisphere, this study aimed to investigate in vitro the toxic effect of these mycotoxins on intestinal...