thymus is a medicinal plant which contains one of the world's top ten essential oils, exhibiting antibacterial, antioxidative, food preservative and mammalian age-delaying properties. this work was aimed at identifying between-species variations requiring for selecting appropriate parents for hybridization. six iranian endemic thymus accessions belonging to thymus daënensis, t. eriocalyx and t....

High-quality DNA extraction plays an important role to make sharp bands in the gel electrophoresis and also produces clean chromatograms. Usually, DNA extract is delivered using the modified CTAB method but this method cannot obtain high-quality DNA for molecular analysis from old dried leaves of Bellevalia due to having different chemical compounds which inhibit to obtain a clear DNA extractio...

In any organism that reproduces sexually, DNA Recombination plays vital roles in the generation of allelic diversity as well as in preservation of genome fidelity. Genome fidelity is particularly important in plants because mutations occurring during the development of flowering plants are heritable and can be passed onto the next generation. One of the gene families that play crucial roles in ...

We used [methyl-(3)H] dimethyl sulfate to probe the genome structures of several RNA and DNA viruses. We compared sites of modification in nucleic acids that were methylated chemically before and after extraction from purified virions. With both single-stranded and double-stranded substrates alkylation occurred mainly at the N7 position of guanine. However, adenine N1 atoms were differentially ...

Kernel-based method is one of the promising algorithms in the pattern recognition field [1]. We have extended the algorithm to the symbolical reasoning tasks (we call the algorithm symbolical kernel-based reasoning (SKBR)) [2], and we currently apply the SKBR to the rule extraction in Dictyostelium discoideum the DNA and cDNA databases of which are well established already [3]. Goal of our proj...

introduction: dna extraction methods are very important for genetics research and diagnostic tests. in these methods are also important for detecting genetic diseases or in cancers for investigating genetic changes during cancer progression or treatment. therefore, selection of the best method for dna extraction from different samples such as bone marrow (bm) or peripheral blood (pb) and their ...

background: fetal dna in maternal plasma and serum has been shown to be a useful material for prenatal fetal sex determination during early gestational ages. non-invasive prenatal diagnosis is now possible at 8th week of pregnancy, by maternal blood sample testing. objective: the purpose of this study was to evaluate two dna extraction methods from mother plasma and its routine clinical applica...

The new coronavirus pandemic brought the need for researchers to work tirelessly in search therapies or vaccines that would allow control of spread virus around world. Fortunately there are two RNA against COVID-19 have finished clinical tests and approved some countries. They first third generation history time record. But still concerns about safety DNA/RNA vaccines. Here we bring a discussio...

AIMS To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA ...