a new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising dna annealing-ligation and augmentation by polymerase chain reaction was introduced. the sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. first, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

background: replacment of cd133+ cell’s β globin gene by using a gene targeting construct containing the β globin gene and essential elements for homologous recombination. materials and methods: pfbggt was amplified, then digested using the nhei and xhoi restriction enzymes, and finally, a 13.3 kb band (naked dna) was extracted from the agarose gel. biological activity of positive and negative ...

BACKGROUND Alpha thalassemia (α-thal) is one of the most common hemoglobinopathies worldwide. The aim of this study was to investigate the spectrum of α-thal mutations among premarital Baluch couples in southeastern Iran. SUBJECTS AND METHODS We assessed 1215 individuals by multiplex gap polymerase chain reaction (gap-PCR) and amplification refractory mutation system (ARMS-PCR). RESULTS Of ...

The inverse relationship between expression and methylation of -type globin genes is well established. However, little is known about the relationship between expression and methylation of avian -type globin genes. The embryonic globin promoter was unmethylated, and -globin RNA was easily detected in 5-day chicken erythroid cells. A progressive methylation of the CpG dinucleotides in the promot...

Introduction: Despite improvements in the diagnosis and treatment of lung cancer in the past two decades, it has remained the most common cause of death from cancer worldwide. Among all genes that are mutated in lung cancer, TP53 located on chromosome 17P13/1 has a significant diagnostic and prognostic value. TP53 mutations have been extensively studied in lung cancer and TP53 mutational spectr...

Bacground: Wilson's disease is a rare autosomal recessive disorder characterized by toxic accumulation of copper in liver and brain. The disorder is caused by mutations in the ATP7B gene, encoding a copper transporting P-type ATPase. Characterization of the spectrum of mutations in this gene is important both for diagnosis and genetic counseling of the families. Materials and Methods: We enrol...

conclusions more studies are needed to investigate the relationship between other parts of this gene with hearing loss in different populations through the country. more research could clarify the role of this gene and its relation with deafness and provide essential information for the prevention and management of auditory disorders caused by genetic factors in the iranian population. backgrou...

High-level -globin expression depends on cis-acting regulatory sequences located far upstream of the -globin cluster. Sequences that contain the -globin positive regulatory element (PRE) activate -globin expression in transgenic mice. The -globin PRE contains a pair of composite binding sites for the transcription factors activating protein 1 and nuclear factor erythroid 2 (AP1/NFE2). To determ...