A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating...

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

Background: Brain tissue segmentation for delineation of 3D anatomical structures from magnetic resonance (MR) images can be used for neuro-degenerative disorders, characterizing morphological differences between subjects based on volumetric analysis of gray matter (GM), white matter (WM) and cerebrospinal fluid (CSF), but only if the obtained segmentation results are correct. Due to image arti...

in recent years, biotechnology has provided the possibility of creating genetically modified (gm) organisms by transferring genes from sources such as viruses, bacteria, animals, and plants into usually unrelated species. this ability to overcome insurmountable physiological barriers by exchanging genetic materials among all living organisms is a double-edged sword. on the one hand, it allows t...

We propose a particle-based distributed PHD filter for tracking the states of an unknown, time-varying number targets. To reduce communication, local filters at neighboring sensors communicate Gaussian mixture (GM) parameters. In contrast to most existing filters, our employs “arithmetic average” fusion. For particles–GM conversion, we use method that avoids particle clustering and enables sign...

studies have shown that aflatoxin is immunosuppressive and its ingestion in feed has resulted in decreased immunity in vaccinated birds .therefore, the purpose of the present study was to determine the efficacy of sodium bentonite (sb) and esterified glucomannan (e-gm) of yeast cell wall, in counteracting the toxic effects of aflatoxin (af) on immunization against infectious bursal disease (ibd...

Gm types of sera from Caucasians and Negroes, of myeloma globulins, and of specific antibodies were investigated. In particular the relationship between the recently identified gene Gm(f) and other Gm genes was analyzed. 1. Caucasians were, with rare exceptions, either Gm(b+f+) or Gm(b-f-). 2. In Negroes, on the other band, type Gm(b+f-) was found to be common. 3. No myeloma globulins appeared ...