A three-laboratory study was performed to evaluate conidium counting in a hematocytometer as a technique of inoculum preparation for susceptibility testing of Aspergillus spp. In addition, inocula were quantified by colony counting and optical density determination. The agreement and correlation coefficient between conidium and colony quantifications were 89.2% and 0.73 (P < 0.01). Correlations...

With the development of the Makler counting chamber, the reproducibility and accuracy of computer-assisted semen analysis and of manual determination of sperm concentration and sperm motility were significantly enhanced beyond what is possible with other methods, such as the hemocytometer, the Horwell chamber, the Petroff-Hausser chamber, and Chartpak slides. The use of Micro-Cell, a disposable...

In this study we investigated the effect of near-infrared fluorescent quantum dots (NIRF-QDs, QTracker) on the proliferation, adherence, invasion and chemotaxis of human tongue squamous cell carcinoma cell line Tca8113 in vitro. Cell proliferation and colony formation rate were determined by using a hemocytometer and culture plate. A transwell chamber assay was used to determine the cell invasi...

Allergic rhinitis (AR) is the chronic type of that arises due to hypersensitivity response. It caused by nasal mucosa inflammation and becomes a social burden in later stages. Ponicidin diterpenoid isolated from herbs like Rabdosia rubescens possesses anti-inflammatory properties. Hence, present study was designed analyze effects against AR using ovalbumin (OVA)-sensitized murine model also rev...

Objective(s) Air pouch is a well-established inflammatory model in which fluid extravasations; leukocyte migration, angiogenesis and other parameters involved in the inflammatory response can be measured. In this study, the influence of gender on inflammatory parameters has been examined in the air pouch model. Materials and Methods To induce air pouch, adult male and female Wistar rats were...

Background: Cutaneous leishmaniasis (CL) remains a major health threatening disease in Iran and many countries around the world. Antimony compounds are currently used to treat CL. Due side effects high resistance, use of alternative therapies, especially nanoparticles, has been considered by researchers. The aim this study was investigate anti-leishmanial activity selenium nanoparticles (SeNPs)...

PURPOSE Dichlorotriazinyl aminofluorescein (DTAF) has been used to stain corneal stromal collagen as part of in vivo experimentation. Toxicity of this drug, if present, might alter the observed wound healing. To determine if this drug has any deleterious effect on keratocytes, we evaluated it in vitro. METHODS Human keratocytes in 96 well plates were exposed to different concentrations of DTA...

Induced sputum cell counts provide a relatively noninvasive method to evaluate the presence, type, and degree of inflammation in the airways of the lungs. Their interpretation requires a knowledge of normal values from a healthy population. The objective was to examine the total and differential cell counts in induced sputum from a sample of healthy adults. A total of 118 healthy nonsmoking adu...

Accurate determination of sperm concentration in fluid suspension is a critical component in a semen analysis. Inaccurate estimations can lead to misinterpretation of the spermiogram and, in the case of livestock production, can lead to faulty insemination doses, which can adversely affect stud power, fertility, fecundity, and cost effectiveness of breeding programs. Capillary-loaded slides, li...

In cnidarian symbiosis research, studying algal uptake, maintenance, and expulsion typically requires quantification of density in host tissue. Multiple methods are used to measure including vitro cell counts holobiont homogenate situ tentacle clippings. The relative precision both types measurement has not previously been reported for the model Exaiptasia diaphana fully symbiotic state. object...