Six natural polyphenolic compounds, brevifolin carboxylic acid, brevifolin, ellagic acid, methyl gallate, gallic acid and protocatechuic acid have been isolated from the methanol extract of the whole plant of Erodium cicutarium (L.) L.'Hérit. (Geraniaceae). Structures were determined by conventional methods of analysis and confirmed by MS and NMR spectral analysis. The distribution of these com...

In this study, we have chosen HPTLC as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homoeopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of Arjunolic acid and fingerprinting of the in-house mother tincture considered here to be a standard with that of different marketed sampl...

Objective: To establish the fingerprint profile of Wedelia chinensis using thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC) technique. Methods: TLC and HPTLC studies were carried out in two different solvent systems, which showed different Rf value. Results: TLC profiling of the extract confirm about the presence of various phytochemicals. HPTLC finger prin...

A quantitative method using silica gel HPTLC plates, automated bandwise sample application, detection with ninhydrin chromogenic reagent solution, and automated visible mode densitometry has been developed for determination of glucosamine in nutritional supplements containing a variety of other active and inactive ingredients. Accuracy was validated by analysis of spiked blank and standard addi...

In this study, we have chosen HPTLC as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homoeopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of BacosideA and fingerprinting of the in-house mother tincture considered here to be a standard with that of different marketed samples av...

Among various meanings of TLC like The Last Cigarette—a smoking cessation program that offers numerous resources and tools to assist smokers in their quit efforts, there is also Thin Layer Chromatography, an analytical technique, which can be useful to check the process of quitting smoking and allow to observe differences in nicotine metabolism. The task of this research was checking sufficienc...

A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report...

A quantitative method using silica gel HPTLC plates, automated band-wise sample application, detection with ninhydrin chromogenic reagent solution, and automated visible mode densitometry has been developed for determination of L-arginine hydrochloride in nutrition supplements containing a variety of other active and inactive ingredients. Accuracy was validated by analysis of spiked blank and s...

A method for the separation and quantification of a complex ganglioside mixture from a clinically available amount (5 ml) of human cerebrospinal fluid (CSF) is described. After reduction of the CSF volume by ultrafiltration, gangliosides are extracted with methanol/chloroform, then separated and quantified by high performance thin layer chromatography (HPTLC) and direct densitometry. For purifi...