The objective of this study was to develop a short-term culture system for buffalo spermatogonial stem cells (SSCs) in various culture conditions. Spermatogonial stem cells as well as sertoli cells were isolated from prepubertal buffalo calf testes and enriched to culture for two weeks in a serum-free medium. The serum-free medium was supplemented with various concentrations of serum (FBS) or f...

Twenty years ago, the transplantation of spermatogonial stem cells (SSCs) from a mouse to other recipient mice was shown to be feasible, which clearly demonstrated the functional identity of SSCs. Since then, several important new findings and other technical developments have followed, which included a new hypothesis on their cell kinetics and spermatogonial hierarchy in the testis, a culture ...

enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (sscs) transplantation. knock-out serum replacement (ksr) has been reported to enhance the proliferation of murine sscs and human embryonic stem cells. the aim of the present study was to investigate the effect of ksr versus fetal bovine serum (fbs) ...

Spermatogonial stem cell transfer has been studied in various species and suggested potential for success. Bull, stallion, donkey, mule, boar, dog and cat testicles were examined using various injection techniques to determine distribution patterns of injected material most favorable for stem cell transfer. Spermatogonial stem cells were recovered from freshly harvested stallion testicles using...

At the apical tip of Drosophila testis, there is a stem cell niche known as the proliferation center, where the stem cells are maintained by hub cell cluster for the regulation of differentiation and proliferation. Germline stem cells go through mitosis four times from one primary spermatogonial cell to the 16-cell stage before the maturation. The cells derived from the same germline stem cell ...

Spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. The present study was designed to examine the effect of hCG treatment on germ cell proliferation following stem cell transplantation in mice. Spermatogonial stem cells were isolated from neonatal mice testes and characterized by alkaline phosphatase, immunoreactivity and ...

BACKGROUND Spermatogonial expansion in man and non-human primates has been studied for decades. Controversy persists about the cell type representing the testicular stem cell and the exact kinetics of spermatogonial proliferation. We recently determined the starting point of spermatogenesis and proposed a model for clonal expansion of spermatogonia in adult macaques. Here we want to confirm the...

BACKGROUND A key step in studying the biology of spermatogonia is to determine their global gene expression profile. However, disassociation of these cells from the testis may alter their profile to a considerable degree. To characterize the molecular phenotype of human spermatogonia, including spermatogonial stem cells (SSCs), within their cognate microenvironment, a rare subtype of human defe...