A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...

In Enterobacteriaceae, the RNA chaperone Hfq mediates the interaction of small RNAs with target mRNAs, thereby modulating transcript stability and translation. This post-transcriptional control helps bacteria adapt quickly to changing environmental conditions. Our previous mutational analysis showed that Hfq is involved in metabolism and stress survival in the enteropathogen Yersinia enterocoli...

Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and div...

Salmonella typhimurium induced massive uptake of extracellular fluid in epithelial cells in the form of macropinosomes. The appearance of macropinosomes in the infected cell was related to the induction of membrane ruffling during bacterial invasion. A noninvasive S. typhimurium invA mutant did not trigger such effects in the host cell. Similarly, S. typhimurium invA mutants that invaded via th...

Le sésame constitue le deuxième produit d’exportation agricole au Burkina Faso mais sa qualité n’est pas toujours satisfaisante à cause de la présence d’impuretés et parfois microorganismes pathogènes notamment les salmonelles. La présente étude a pour objectif d’évaluer prévalence des salmonelles dans ses produits dérivés. Au total, 107 échantillons dont 73 graines 34 dérivés (biscuits, croque...

ABSTRACT Salmonella is a foodborne pathogen that has contributed to numerous food safety accidents worldwide, making it necessary detect contamination at an early stage. A pair of specific primers based on the invA gene was designed for PCR. Target double-stranded DNA (dsDNA) from PCR purified and denatured high temperature obtain target single-stranded (ssDNA). Two carbo...