A simple and rapid luminometric assay for the detection of chemical inhibitors of human thyroid peroxidase (hTPO) activity was developed and validated with 10 model compounds. hTPO was derived from the human thyroid follicular cell line Nthy-ori 3-1 and its activity was quantified by measuring the oxidation of luminol in the presence of hydrogen peroxide (H2O2), which results in the emission of...

OBJECTIVE: This study was designed to test two kinds of latent blood reagents, luminol and Bluestar® Magnum, and to determine their ability to detect latent blood and to preserve DNA for genetic profile determinations. DESIGN: Three dilutions of blood were applied to six substrates: vinyl tile, ceramic tile, treated and untreated wood, carpet and cinderblock. Each blood dilution, 1:1, 1:10 and ...

The possibility that the mode of action of sulphasalazine and its active metabolite 5-amino-salicylic acid (5ASA) involves modification of toxic oxygen metabolite production by neutrophils has been investigated by measuring the effect of these drugs on luminol-dependent chemiluminescence, superoxide release and oxygen consumption by stimulated neutrophils in vitro. 5ASA, and to a lesser extent ...

The relationship between differential cell counts and latex-stimulated luminol and lucigenin-amplified chemiluminescence (CL) was investigated by mixing alveolar macrophages (AM) obtained at bronchoalveolar lavage (BAL) with allogeneic peripheral blood neutrophils (PMN) in varying proportions. In 5 non-asthmatic subjects, the mean luminol-amplified CL increased linearly from 2.1 (0.9 SEM) x 10(...

We report a novel and facile electrodeposition method to fabricate a nano-structure film of the unsubstituted metal phthalocyanine on a glassy carbon electrode (GCE). In this electrodeposition system, unsubstituted iron(II) phthalocyanine (u-FePc) was chosen as the model complex of the unsubstituted metalphthalocyanine, and the ionic liquid 1-octyl-3-methylimidazolium trifluoroacetate was emplo...

A flow injection system with chemiluminescence detection for the determination of asulam, enhancer of the system luminol-H(2)O(2)-horseradish peroxidase, is proposed. The method shows a moderate selectivity against other pesticides usually present in formulations of herbicides and in water. The procedure was applied to the determination of asulam in tap water samples and a recovery study was ca...

This study is based on a simple chemical interaction of peroxynitrite (O = N-O-O-) and luminol, which produces blue light upon oxidation. Since peroxynitrite has a half-life of about 1 s, a drug known as linsidomine (SIN-1) is used as a peroxynitrite generator. Peroxynitrite can oxidize lipids, proteins and nucleic acids. Upon the stimulation of inflammation and/or infection, macrophages and ne...

The article shows the antioxidant properties of new derivatives 2-aminothiazole containing diterpene fragment. Using method luminol-dependent chemiluminescent analysis it is revealed that tested compounds under investigation exhibit in model test systems, where processes reactive oxygen species formation, lipid peroxidation, and cell phagocytosis blood are induced.