Normal human peripheral blood lymphocytes cultured with phytohemagglutinin in folate-free RPMI 1640 medium supplemented with normal human serum exhibit megaloblastic maturation. These changes are accompanied by a decrease in intracellular folate content, and when compared to folate-replete cells, reveal increased 3H-thymidine incorporation into DNA together with a decreased inhibitory effect of...

BACKGROUND Anemia is a common accompaniment of cerebral palsy, mental retardation and neurodegenerative disorders. CLINICAL CHARACTERISTICS A 4-year-old boy with chronic megaloblastic anemia, global developmental delay, seizures, intracranial calcification and new onset neuro-regression. OBSERVATION A diagnosis of hereditary folate malabsorption was made, and he was put on oral and injectab...

Anemia is one of the most common health problems in the primary care setting. Macrocytosis in adults is defined as a red blood cell (RBC) mean corpuscular volume (MCV) >100 femtoliter (fL). Macrocytic anemias are generally classified into megaloblastic or nonmegaloblastic anemia. Megaloblastic anemia is caused by deficiency or impaired utilization of vitamin B12 and/or folate, whereas nonmegalo...

Objective: To determine the frequency of different causes of pancytopenia on bone marrow examination. Study Design: Descriptive study. Place and Duration of Study: The study was carried out at Haematology (pathology) department of Army Medical College, National University of Sciences and Technology (NUST) and Military Hospital Rawalpindi from Jan 2012 – Dec 2012. Patients and Methods: Total 67 ...

Recent studies using anion exclusion chromatography have suggested that uracil is misincorporated into the DNA of patients with megaloblastic anemia to levels detectable by nonradioactive methods. We have investigated the nucleotide composition of DNA from the bone marrow mononuclear cells of eight patients with cobalamin deficiency and compared this with that found in normal subjects. The medi...

Folic-acid deficiency may arise in conditions which produce an increased demand for the vitamin. Diseases in this category include acute leukaemia, disseminated carcinomatosis (Girdwood, 1959), myelofibrosis (Chanarin, Mollin, and Anderson, 1958), and haemolytic anaemia (Chanarin, Dacie, and Mollin, 1959). The presence of megaloblastic erythropoiesis in myelofibrosis has been described in only ...