Intracellular thermometry at the microscopic level is currently a hot topic. Herein we describe a small molecule fluorescent thermometer targeting mitochondria (Mito thermo yellow). Mito thermo yellow successfully demonstrates the ability to monitor the intracellular temperature gradient, generated by exogenous heating, in various cells.

BACKGROUND The ATP-sensitive potassium channel (K(ATP)) opener nicorandil used instead of potassium in hypothermic cardioplegia significantly improves preservation of cardiac function and energetics in the in situ heart preparation. The present study, therefore, examines the effect of nicorandil at different temperatures and the role of sarcolemmal and mitochondrial K(ATP) channels under ex viv...

The creatine kinase (CK) system is involved in the rapid transport of high-energy phosphates from the mitochondria to the sites of maximal energy requirements such as myofibrils and sarcolemmal ion pumps. Hearts of mice with a combined knockout of cytosolic M-CK and mitochondrial CK (M/Mito-CK(-/-)) show unchanged basal left ventricular (LV) performance but reduced myocardial high-energy phosph...

ion Level RULE-SCHEMA: MENINGITIS.COVERFOR.CLINICAL RULE-MODEL: COVERFOR-IS-MODEL KEY-FACTOR: BURNED DUAL: D-RULE577

During the course of a screening program for antibiotics produced by a Saccharothrix, two new kinamycins 1 and 2 have been isolated from cultured broth of strain MI293-N4. They inhibit the growth of Gram-positive bacteria and neoplastic cells. This paper describes the production, isolation, purification and the structure elucidation of the antibiotics. Some physicochemical properties and biolog...

Patients with relapsed acute myeloid leukemia (AML) have unfavorable prognosis and require innovative therapeutic approaches. In this study we used fludarabine combined with a middle dose of cytosine arabinoside (Ara-C), mitoxantrone and granulocyte-colony stimulating factor (G-CSF) as a salvage therapy for patients with relapsed AML in China. Forty-five patients with relapsed AML were treated ...

I review the research leading from the GUIDON rule-based tutoring system, including the reconfiguration of MYCIN into NEOMYCIN and NEOMYCIN’s generalization in the heuristic classification shell, HERACLES. The presentation is organized chronologically around pictures and dialogues that represent conceptual turning points and crystallize the basic ideas. My purpose is to collect the important re...

Chemo-resistance is a clinical barrier to more effective anti-cancer therapy. In this context, cancer stem-like cells (CSCs) are thought to be chemo-resistant, resulting in tumor recurrence and distant metastasis. Our hypothesis is that chemo-resistance in CSCs is driven, in part, by enhanced mitochondrial function. Here, we used breast cell lines and metastatic breast cancer patient samples to...

As described in Part Eight, the success of MYCIN as a problem solver suggested that the program’s knowledge base might be a suitable source of subject material for teaching students. This use of MYCIN was consistent with the design goals that the program’s explanations be educational to naive users and that the representation be flexible enough to allow for use of the rules outside of the consu...