Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

managers in the organization require the right information to decide. sometimes those around the manager may avoid providing correct but disturbing information to the manager who may be deceived by the false praise. flattery destroys confidence and demeans the flattered. it also hurts the flatterer by destroying his or her credibility and integrity. in islamic and literary texts there are many ...

Some people contend t h a t fetuses have moral s ta tus but less t h a n t h a t of paradigm persons. Many people hold views implying that sent ient animals have moral s t a t u s but less t h a n that of persons. These positions suggest tha t moral s ta tus admits of degrees. Does it? To address this question, we must first clarify what it means to speak of degrees of moral status. The paper b...

John Hare has proposed “prescriptive realism” in an attempt to stake out a middle-ground position in the twentieth century Anglo-American debates concerning metaethics between substantive moral realists and antirealistexpressivists. The account is supposed to preserve both the normativity and objectivity of moral judgments. Hare defends a version of divine command theory. The proposal succeeds ...

in the middle  of  the  historic  resourc e، shouldn’t  forget  importanc e  of  literaries    that  in  addition  to have  report  of  historic  events ، did  intellectual  thoughts  that  it ‘s possitive  effect  on   human  life and correction of people‘s personality is undeniable. beyhaqi‘s  history   is  one  of   the   dearest  literaries  that  have  big  sowing   form  amsal  va  hekam ...

Could an ethical theory ever play a substantial evidential role in a scientific argument for an empirical hypothesis? In The Descent of Man, Darwin includes an extended discussion of the nature of human morality, and the ethical theory which he sketches is not simply developed as an interesting ramification of his theory of evolution, but is used as a key part of his evidence for human descent ...