نتایج جستجو برای: mouse embryonic stem cells
تعداد نتایج: 1704660 فیلتر نتایج به سال:
Purpose: Determination of changes of glycoconjugates, which have special terminal sugars, in embryonic developmental processes of brain stem and trigeminal nucleus in Balb/C mouse. Materials and Methods: In this study mice embryos between 10-17 embryonic days were used. They were fixed according to ordinary laboratory procedures. The specimens were embedded in paraffin and serial sections with...
Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripotent stem cells, respectively. Although a significantly higher chimera rate re...
background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...
background: the present study was designed to evaluate the homing potential of mouse embryonic stem cells (esc) treated with erythropoietin (epo) in hematopoietic organs such as spleen and liver after transplantation using morphological and immuno-histochemical techniques. methods: day-four embryoid body (eb)-derived cells were dissociated and re-plated in medium in the presence and absence of ...
stem cells are the cells having some distinguishing characteristics like longevity, high capacity of self-renewal and differentiation, quiescence and highly error-free proliferation. almost all stem cells have the potential of lineage reprogramming, i.e. inter-conversion of cell lineages. they also have the potential to differentiate into almost all kinds of cells. these cells have been found i...
Introduction: Contrary to a common belief, most mammalian females lose the ability of Germ Cell (GC) renewal and oogenesis during fetal life. Although, it has been claimed that germ line stem cells preserve oogenesis in postnatal mouse ovaries, that postnatal oogenesis keeps producing functional and sufficient GCs in the case of infertility (caused by different reasons) is doubtful. On the othe...
Purpose: We investigated the effect of (bFGF) (basic-Fibroblast Growth Factor) on the differentiation of divided cardiomyocytes from mouse embryonic stem cells (ES) and their pharmacological properties. Materials and Methods: The mouse embryonic stem cells (Royan B1) were cultured as 800 cells per 20µl of a hanging drop. After two days, ES cells in each drop aggregated to form embryoid bodies ...
stem cells are undifferentiated biological cells that can differentiate into specialized cells and can divide (through mitosis) to produce more stem cells. they are found in multicellular organisms. in mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in various tissues. in ad...
Purpose: Isolation, induction of neural and glial differentiation and evaluating the expression of Nucleostemin, ZFX, Hoxb-4, Sox-9 & Bmi-1 self renewal genes in adult mouse neural stem cells. Materials and Methods: Breifly, for isolation of neural stem cells, frontal part of adult mouse brain was minced in PBS and digested by enzyme solution, containing hyaloronidase and trypsin. Isolated cel...
Stem cells are undifferentiated biological cells that can differentiate into specialized cells and can divide (through mitosis) to produce more stem cells. They are found in multicellular organisms. In mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in various tissues. In ad...
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