BACKGROUND Viral hemorrhagic fevers (VHFs) are a group of animal and human illnesses that are mostly caused by several distinct families of viruses including bunyaviruses, flaviviruses, filoviruses and arenaviruses. Although specific signs and symptoms vary by the type of VHF, initial signs and symptoms are very similar. Therefore rapid immunologic and molecular tools for differential diagnosis...

We developed a highly sensitive reverse transcription and multiplex real-time PCR (rtPCR) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups I and II; sapovirus genogroups I, II, IV, and V; human rotavirus A; adenovirus serotypes 40 and 41; and human astrovirus. In comparison to monoplex rtPCR assays, the sensitivities and specificities of...

A new method based on least square support vector machine (LSSVM) combined with FastICA is proposed to extract the fetal electrocardiogram (FECG) from the abdominal signals of a pregnant woman. Firstly, the LSSVM is applied to estimate the maternal electrocardiogram (MECG) component in the multiplex abdominal signals. Then the optimal estimation of multiplex noise-added FECG is obtained by remo...

Motivated by online social networks that are linked together through overlapping users, we study the influence maximization problem on a multiplex, with each layer endowed with its own model of influence diffusion. This problem is a novel version of the influence maximization problem that necessitates new analysis incorporating the type of propagation on each layer of the multiplex. We identify...

SYBR Green I (SG) is widely used in real-time PCR applications as an intercalating dye and is included in many commercially available kits at undisclosed concentrations. Binding of SG to double-stranded DNA is non-speci®c and additional testing, such as DNA melting curve analysis, is required to con®rm the generation of a speci®c amplicon. The use of melt curve analysis eliminates the necessity...

SYBR Green I (SG) is widely used in real-time PCR applications as an intercalating dye and is included in many commercially available kits at undisclosed concentrations. Binding of SG to double-stranded DNA is non-specific and additional testing, such as DNA melting curve analysis, is required to confirm the generation of a specific amplicon. The use of melt curve analysis eliminates the necess...

Sensitive and accurate identification of specific DNA mutations can influence clinical decisions. However accurate diagnosis from limiting samples such as circulating tumour DNA (ctDNA) is challenging. Current approaches based on fluorescence such as quantitative PCR (qPCR) and more recently, droplet digital PCR (ddPCR) have limitations in multiplex detection, sensitivity and the need for expen...