background and objectives: we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. materials and methods: the specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. in total, 193 clinical isolates were ...

background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...

purpose: the main purpose of this study was to evaluate the prevalence of herpes simplex virus 1 and 2 (hsv-1 and hsv-2), varicella-zoster virus and adenovirus associated with conjunctivitis in swab samples of patients who referred to eye specialist hospitals in tehran. methods: in this cross-sectional study, swab samples from patients with acute conjunctivitis during the first sixth months of ...

background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...

conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...

background and objective: diarrheal disease is still a major health problem, especially in developing countries, where it is considered as one of the leading causes of morbidity and mortality especially in children. studies showed that diarrheagenic e. coli (dec) such as stes and epec strains are among the most prevalent causative agents in acute diarrhea, particularly in children. aim of the p...

Salmonella (S.) Typhimurium and S. Enteritidis are the major causative agents of food-borne illnesses worldwide. Currently, a rapid detection system using multiplex real-time polymerase chain reaction (PCR) has been applied for other food-borne pathogens such as Escherichia coli, Staphylococcus aureus and Streptococcus spp. A multiplex real-time PCR was developed for the simultaneous detection ...

We extend the observability model to multiplex networks. We present mathematical frameworks, valid under the treelike ansatz, able to describe the emergence of the macroscopic cluster of mutually observable nodes in both synthetic and real-world multiplex networks. We show that the observability transition in synthetic multiplex networks is discontinuous. In real-world multiplex networks instea...