purpose: to ascertain whether the use of nested-pcr (nested polymerase chain reaction) technique leads to more rapid and accurate diagnosis of bacterial endophthalmitis. methods: the aqueous humor and vitreous samples of 24 eyes of 24 patients with bacterial endophthalmitis were evaluated by microscopy, diagnostic aerobic culture, and nested- pcr to detect the infectious agent. results: aerobic...

conclusions the study indicated that the rate of p. jirovecii infection is similar to those reported from asian, indian and african countries. the similarity of the genetic pattern between iranian and indian isolates is probably due to their close geographical proximity. patients and methods we used two nested pcr and pcr-rflp assays to amplify mt lsu rrna and dhps genes in 126 samples obtained...

cryptosporidiosis is a gastroenteric disease caused by the protozoan parasite cryptosporidium parvum. water–borne transmission of this organism has become more prevalent in recent years. current method for detection of c. parvum oocysts in water is immunofluoresence assay (ifa). the method is time consuming, laborious and particularly not–specific. it cannot determine the infectivity of detecte...

In vitro gene chemical synthesis is a powerful tool to improve the expression of gene in heterologous system. In this study, a two-step gene synthesis strategy that combines an assembly PCR and an overlap extension PCR (AOE) was developed. In this strategy, the chemically synthesized oligonucleotides were assembled into several 200-500 bp fragments with 20-25 bp overlap at each end by assembly ...

BACKGROUND & OBJECTIVE The expression of human telomerase reverse transcriptase (hTERT) is positively correlated to the activity of telomerase. Alternative splicing exists in the transcription of hTERT and special splicing patterns may change during tumor progression. This study was to reveal the changes of hTERT alterative splicing pattern in gastric carcinogenesis. METHODS Three alternative...

The aim of this study was to construct a human single-chain variable fragment (scFv) gene library for gastric cancer, from which human anti-cancer-associated gene (CAGE) scFvs are selected. Human lymphocytes were isolated from the peripheral blood of 10 gastric cancer patients and whole human heavy and light chain genes were cloned by reverse transcription-polymerase chain reaction (RT-PCR). VH...