Background: Diabetes mellitus as a chronic disease can cause fertility problems in male human population. In fact, it exerts sever damages in testicular tissue and subsequently reduces total number of normal sperms and semen volume. Therefore, this study is designed to evaluate the protective effect of Equisetum arvense extract on sperm quality, DNA damage and maturation in diabetic male mice. ...

DNA synthesized by particulate fractions from human leukemic white blood cells has been subfractionated by hybridization to Rauscher leukemia virus 70S RNA followed by hydroxylapatite chromatography. The Rauscher-leukemia-virus-specific DNA fraction is shown to be complementary only to sequences present in the nuclear DNA of Balb/c mouse spleens infected by this virus and the nuclear DNA from h...

BACKGROUND High throughput sequencing (HTS) technologies have revolutionized the field of genomics by drastically reducing the cost of sequencing, making it feasible for individual labs to sequence or resequence plant genomes. Obtaining high quality, high molecular weight DNA from plants poses significant challenges due to the high copy number of chloroplast and mitochondrial DNA, as well as hi...

Nuclear import of plasmid DNA in nondividing cells is a process essential to the success of numerous viral life cycles, gene therapy protocols, and gene expression experiments. Here, intact protein-free SV40 DNA was cytoplasmically injected into cells and its subcellular localization was followed by in situ hybridization. SV40 DNA localized to the nucleus consistent with a mechanism of transpor...

Background: About 15% of male infertility is due to genital tract infection and inflammation, some them have no clinical symptoms, but manifested as leukocytospermia (LCS). Leukopenia will lead functional impairment sperm integrity damage morphology. A large amount reactive oxygen species (ROS) produced by leukocytes can nuclear DNA. The aim this study was investigate the correlation between le...

We have examined the time course and patterns of covalent aflatoxin B^DNA adducts produced within the ribosomal RNA gene sequences isolated from the liver nuclear DNA of aflatoxin Bi-treated animals. Liver nuclear DNA was initially enriched in ribosomal DNA by cesium salt density centrifugaron, and incu bated under alkaline conditions to stabilize bound aflatoxin ST ONA moieties. Alkali-treated...

The mitochondria and plastids of eukaryotic cells evolved from endosymbiotic prokaryotes. DNA from the endosymbionts has bombarded nuclei since the ancestral prokaryotes were engulfed by a precursor of the nucleated eukaryotic host. An experimental confirmation regarding the molecular mechanisms responsible for organelle DNA incorporation into nuclei has not been performed until the present ana...