نتایج جستجو برای: pcr analysis to extract total rna

تعداد نتایج: 11639216  

Journal: :مجله بین المللی علوم آزمایشگاهی 0
arina monazah mehdi zeinoddini alireza saeeidinia

backgrounds and aims: coxsakievirus b3 (cvb3), one of the six coxsakievirus b serotypes, is a member of the enterovirus genus within the picornaviridae family. cvb3 is an important pathogen of viral myocarditis, which accounts for more than 50% of viral myocarditis cases. the genome of cvb3, like that of other entroviruses, is a single-stranded, sense, polyadenylated rna molecule with 7400 nucl...

Objective: We examined the effectiveness of Hyssopus officinalis (hyssop) aqueous extract on pentylenetetrazole (PTZ)-induced acute seizures and the hippocampus iNOS (induciblenitric oxide synthases) gene expression as a potential mediator of the effects. Materials and Methods: Adult male Wistar rats were used. Tonic-clonic seizures were...

M. Roostaei-Ali Mehr, R. Motamedi-Mojdehi R. Rajabi-Toustani

Leptin is secreted mainly by fat, which is involved in energy metabolism and reproduction. Leptin and itsreceptor (Ob-R) have been detected in human spermatozoa and testis, thus it can be concluded leptin involves in male physiology. The goal of this study was determination of the presence of leptin receptor mRNA in the ram testis, epididymis and spermatozoa by reverse transcription polymerase ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید چمران اهواز - دانشکده ادبیات و علوم انسانی 1386

the purpose of the present study is to find out whether bilinguals of khuzestan-arab origin or monolinguals of iranian origin code-switch during learning or speaking english and which group is more susceptible to code-switch. to this end, the students of 24 classes from high schools and pre- university centers were screened out, and interviewed and their voices and code-switchings were recorded...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده منابع طبیعی و علوم دریایی 1388

this research was conducted in two protect and destroy region in the middle zagros, in illam province. in order to identification of ecological species group and evaluate density of regeneration, effect of many factors such as protection, vegetation, physiographic factors, physical and chemical properties of soil in study locations were studied. to achieve these purpose number of 54 plots using...

Journal: :BioTechniques 2004
Alan Dafforn Pengchin Chen Glenn Deng Michael Herrler Dawn Iglehart Sriveda Koritala Susan Lato Susheela Pillarisetty Reshma Purohit Martin Wang Shenglong Wang Nurith Kurn

Gene expression analysis has become an invaluable tool for understanding gene function and regulation. However, global expression analysis requires large RNA quantities or RNA preamplification. We describe an isothermal messenger RNA (mRNA) amplification method, Ribo-SPIA, which generates micrograms of labeled cDNA from 5 ng of total RNA in 1 day for analysis on arrays or by PCR quantification....

ژورنال: بیماری های پستان 2021

Introduction: Breast cancer is the most important cause of cancer mortality among women, therefore the study of its causative or aggravating factors seems necessary. In this study, the mRNA levels of the PHB2 gene were evaluated in tumor and adjacent non-tumor tissues of 50 women diagnosed with invasive ductal carcinoma of the breast. Methods: RNX-Plus solution was used to isolate total RNA fro...

Journal: :iranian journal of pathology 0
mehdi nassiri university of miami, jackson memorial hospital, miami, florida mehrdad nadji university of miami, jackson memorial hospital, miami, florida

the rapidly expanding fields of pharmacogenomics and pharmacodiagnostics have presented the pathology laboratories with many challenges and opportunities. as custodians of patient tissues, these laboratories are in the logical position to perform biomolecular testing for proper management of patients. in order to meet these challenges, the pathology laboratories of the twenty-first century shou...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت معلم تهران 1381

‏‎the purpose of the present study was to investigate the latent pattern underlying reading ability . some 272 male and female participants. english majors at the ba level , participated in the study. two valid tests were used in this study, the reading test developed exclusively for the purpose of this project and academic reading section of ielts. to investigate the possible latent underlying...

پایان نامه :دانشگاه تربیت معلم - سبزوار - دانشکده علوم انسانی و پایه 1393

burnout is a response to the chronic work stress which is prevalent mostly among the people who do people job, like teaching. the purpose of this study was to develop a valid and reliable instrument that can measure burnout in foreign language teachers. although some widely used instruments were developed before which measured burnout in teachers, a specific instrument which include specific sy...

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