ObjectivesTo our knowledge no previous study has assessed the performance of a rapid antigen diagnostic immunoassay (RAD) conducted at point care (POC). We evaluated Panbio™ COVID-19 Ag Rapid Test Device for diagnosis coronavirus 2019 disease (COVID-19) in symptomatic patients (n = 412) attending primary healthcare centres.MethodsRAD was performed immediately after sampling following manufactur...

Background and Aims: Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory and kidney disease of chickens which results in significant economic losses in commercial broilers, layers and breeders. Rapid identification of IBV serotypes involved in respiratory complex is a problem in the differential diagnosis. Materials and Methods: In this study, a single and mul...

background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...

human growth hormone (hgh) genomic sequence containing 5 exons and 4 introns was cloned in pcdna-3 and the constructed plasmid was subsequently used for transfection ofnlli-3t3 cell line using lipofection technique. expression of hgh in stably transfected cells was assayed using elisa. total rna was extracted from transfected cells and hgh cdna was amplified by rt-pcr using specific primers. th...

Abstract Severe acute respiratory syndrome coronavirus 2 causing disease 2019 pandemic is an enveloped virus, showing genome similarity with bat coronavirus. This virus initially infects the upper tract, subsequent spread to lower tract. Despite availability of antigen and antibody detection methods, reverse transcription-polymerase chain reaction (RT-PCR) diagnostic test choice for this novel ...

To assess the performance of second reading chest compute tomography (CT) examinations by expert radiologists in patients with discordance between reverse transcription real-time fluorescence polymerase chain reaction (RT-PCR) test for COVID-19 viral pneumonia and CT report. Three hundred seventy-eight were included this retrospective study (121 women 257 men; 71 years median age, a range 29–93...