during 2010-2011, real-time pcr procedure was used to detecting fmdv rna on 147 epithelium samples from the field. in this survey, for real-time pcr from 3d gene segment as conserve region selected for tracking all of seven serotypes fmdv. the assay detected the viral rna in all serotypes of fmdv. the rrt-pcr specifically detected fmd virus in sample with greater sensitivity than our convention...

I n the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in vivo, and the study of virus-host relations...

the invention of polymerase chain reaction (pcr) has been a milestone in the history of biological and medical sciences. the applications of pcr have not only completely revolutionised the research in the field of molecular genetics as well as animal and plant biotechnology, but the technique has also proved its relevance and ingenious utility in other fields of forensic sciences, molecular sys...

soybean mosaic virus (smv) which belongs to the virus family potyviridae, causes a disease in soybean that is present in soybean-growing areas of the world, and is widely distributed in northern iran. detection of smv is very important for disease management. in the present study several serological and molecular (nucleic acid- based) methods of rapid virus detection were compared. serological ...