نتایج جستجو برای: peroxidase enzyme
تعداد نتایج: 265176 فیلتر نتایج به سال:
This article presents a reliable, effective and easy procedure for measuring glutathione peroxidase (Gpx) activity. Enzyme samples were incubated with phosphate buffer, which included the appropriate concentrations of (GSH) peroxide as substrates, to determine Gpx In CUPRAC method, reagent Cu(Nc)22+ was added stop enzymatic reaction after sufficient period incubation. The unreacted substrates a...
T HE ENZYME glutathione peroxidase appears to play a major role in the red cell in the detoxification of potential oxidizing agents. A genetically determined partial, or presumably heterozygous, deficiency of this enzyme has been associated with a transient hemolytic anemia and hyperbilirubinemia in newborns.l* Unless subjected to oxidative stress, red cells from older children and adults with ...
Abstract Background and objective: Exposure of red blood cells to oxygen radicals can induce Lipid proxidation, hemoglobin damage and hemolysis of erythrocyte .The present study was designed to determine the alteration of plasma lipid peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities in stored blood and to find out the quantitative alterations and th...
Background and purpose: Enzymatic treatment, due to various benefits, has attracted many researchers since long time ago. Anionic detergents are one of the largest families of detergents that entered into the environment in recent decades. This study investigated the effect of horseradish peroxidase enzyme process on the removal of linear alkaline benzyl sulfonate (LAS) from aqueous solutions. ...
1. A method is described for the colorimetric determination of peroxidase with N,N-dimethyl-p-phenylenediamine. The amount of red pigment formed by peroxidase is proportional to the concentration of enzyme and to the time of incubation during the first 40 to 90 seconds. The influence of the concentration of enzyme, N,N-dimethyl-p-phenylenediamine, H(2)O(2), the time of incubation, pH, the tempe...
Hydrogen peroxide is a very reactive byproduct of many metabolic pathways. We describe an ultra-sensitive colorimetric method to detect hydrogen peroxide based on the reconstitution of apo-horseradish peroxidase with the hemin derivative, hemin di(N,N'-acetyl-hydrazide). Oxidation of the latter by hydrogen peroxide yields hemin, which is able to reconstitute apo-horseradish peroxidase, forming ...
Peroxidase activity in the seed coats of soybean (Glycine max [L.] Merr.) is controlled by the Ep locus. We compared peroxidase activity in cell-free extracts from seed coat, root, and leaf tissues of three EpEp cultivars (Harosoy 63, Harovinton, and Coles) to three epep cultivars (Steele, Marathon, and Raiden). Extracts from the seed coats of EpEp cultivars were 100-fold higher in specific act...
Abstract In order to evalution of antioxidant enzymes activity and yield of flower of chamomile genotypes under drought stress condition and foliar application of nano iron chelated, a factorial experiment in a randomized complete block design with three replications was conducted at greenhouse research farm of the University of Zabol in 2014. Experimental treatments included drought stres...
A cDNA clone encoding a manganese-dependent peroxidase from the filamentous fungus Phanerochaete chrysosporium was isolated and characterized. The clone, AMP-1, was isolated by screening a X g t l l expression library with polyclonal antibodies raised against a purified manganese-dependent peroxidase (isozyme H4, PI 4.5). The XMP-1 cDNA sequence predicts a mature protein containing 358 amino a...
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