A break in the integrity of the plasma membrane immediately compromises this structure’s essential role as a barrier, and this can kill the affected cell. Yet animal cell plasma membranes, unprotected by a cell wall, are highly vulnerable to mechanically induced disruption. Moreover, many tissue environments generate and receive “physiological” levels of mechanical force that impose shear, tens...

plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence o...

Pore-forming toxins (PFTs) secreted by pathogenic bacteria are the most common bacterial protein toxins and are important virulence factors for infection. PFTs punch holes in host cell plasma membranes, and although cells can counteract the resulting membrane damage, the underlying mechanisms at play remain unclear. Using Caenorhabditis elegans as a model, we demonstrate in vivo and in an intac...

Insulin is present in the seminal plasma and involved sperm activities like motility capacitation. However, effects of insulin on viability cooled ram are not fully understood. Therefore, objective current study was to evaluate effect addition maintained at 5ºC. Sperm samples were collected from six healthy, mature Santa Inês rams. The ejaculates divided into two aliquots with (insulin group) o...

Developing effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed evaluate the effect on sperm kinetic parameters membrane integrity donkey diluted with defined milk proteins extender 1% or 2% of egg yolk removal seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into fo...

Excitatory amino acid receptor activation and reactive oxygen species production are important mediators of neuronal death following acute brain injury and can lead to loss of membrane integrity. Poloxamer 188 (P188) is an amphiphilic, polyethylenepolypropylene-polyethylene copolymer that restores plasma membrane integrity of nonneuronal cells following membrane injury. Here, we demonstrate tha...

in the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. the cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. sperm fertility potential was tested...

Background: Frozen-thawed rooster semen is not reliable for use in artificial insemination in commercial stocks. Low density lipoprotein (LDL) has been assessed for effectiveness as a cryoprotectant in the extender to improve the quality of frozen-thawed rooster semen. Although LDL has been evaluated in a few studies in other species for semen cryopreservation, to date, no study has been conduc...