Peptidase D is one of at least six peptide-hydrolyzing enzymes of Escherichia coli (9). By sequence analysis of the 5' flanking and amino-terminal regions of the corresponding gene, pepD, we were able to show that it is contiguous to the adjacent gpt locus (4). Combination of this sequence with the already-known sequences of the gpt (10), phoE (11), and proBA (2) genes and of two additional ope...

Mutants of Escherichia coli defective in septum initiation, as well as in septum formation were obtained spontaneously, without mutagenic treatment, by selection of rifampin-tolerant mutants of an antibiotic-permeable strain carrying the envA mutation. The disturbed phenotype was in all mutants aggrevated the low incubation temperatures. One allele, sefA1, was studied in detail. Septum initiati...

Intracellular accumulation of the amino acid proline has previously been linked to the salt tolerance and virulence potential of a number of bacteria. Taking advantage of the proBA mutant Escherichia coli CSH26, we identified a listerial proBA operon coding for enzymes functionally similar to the glutamyl kinase (GK) and glutamylphosphate reductase (GPR) enzyme complex which catalyzes the first...

Transduction of R factor 222, which carries four drug resistance markers (~0, strR, camR, totR), by phage P22 in Salmonella typhimurium results in the segregation of the drug resistance markers into tetR and the other three. The tetR marker was found to be integrated at proA near the P22 prophage attachment site of the recipient chromosome, confirming the previous result of Dubnau and Stocker (...

We compared the transcriptome, proteome, and nucleotide sequences between the parent strain Escherichia coli W3110 and the L-threonine-overproducing mutant E. coli TF5015. DNA macroarrays were used to measure mRNA levels for all of the genes of E. coli, and two-dimensional gel electrophoresis was used to compare protein levels. It was observed that only 54 of 4,290 genes (1.3%) exhibited differ...

We isolated 15 mutants of Pseudomonas aeruginosa PAO which were defective in the formation of certain extracellular proteins, such as elastase, staphylolytic enzyme, and lipase ( Xcp mutants). The mutations were mapped on the chromosome by conjugation and transduction. The locations were xcp -1 near 0', with the gene order cys-59- xcp -1- proB , and loci xcp -2, xcp -3, and xcp -31 at 35', with...

An improved, simplified, and greatly shortened isolation procedure for metallothionein from tissue extracts using gel filtration (Sephadex G-SO) and DEAE-cellulose chromatography is presented. When isolated by this procedure, the molecular weight of both the liver and kidney metalloproteins is found to be about 6600, as determined by equilibrium centrifugation and gel filtration. The amino acid...

A new styracosternan ornithopod genus and species is here described based on a partial postcranial skeleton and an associated dentary tooth of a single specimen from the Arcillas de Morella Formation (Early Cretaceous, late Barremian) at the Morella locality, (Castellón, Spain). Morelladon beltrani gen. et sp. nov. is diagnosed by eight autapomorphic features. The set of autapomorphies includes...