نتایج جستجو برای: probe sonication
تعداد نتایج: 96481 فیلتر نتایج به سال:
A chemiluminescent DNA probe (Accuprobe) assay developed by Gen Probe, Inc., for the rapid identification of Histoplasma capsulatum was evaluated and compared with the exoantigen test by using 162 coded cultures including Histoplasma capsulatum var. capsulatum, Histoplasma capsulatum var. duboisii, Histoplasma capsulatum var. farciminosum, Blastomyces dermatitidis, Coccidioides immitis, Paracoc...
In this article a comparative study for modeling and optimization of 2-methylpropane-2-thiol removal from contaminated soil by ultrasound is investigated. Central Composite Design (CCD) and artificial neural network (ANN) were utilized and compared to each other in order to obtain appropriate predicting model with respect to sonication power (w), sonication time (min) and water/reactor volume r...
The microbiological diagnosis of periprosthetic joint infection (PJI) is crucial for successful antimicrobial treatment. Cultures have limited sensitivity, especially in patients receiving antibiotics. We evaluated the value of multiplex PCR for detection of microbial DNA in sonication fluid from removed orthopedic prostheses. Cases of PJI in which the prosthesis (or part of it) was removed wer...
Background—Electrophysiological cardiac devices are increasingly used. The frequency of subclinical infection is unknown. We investigated all explanted devices using sonication, a method for detection of microbial biofilms on foreign bodies. Methods and Results—Consecutive patients in whom cardiac pacemakers and implantable cardioverter/defibrillators were removed at our institution between Oct...
Microorganisms are able to form biofilms within respiratory secretions. Methods to disaggregate such biofilms before utilizing standard, rapid, or high throughput diagnostic technologies may aid in pathogen detection during ventilator associated pneumonia (VAP) diagnosis. Our aim was to determine if sonication of endotracheal aspirates (ETA) would increase the sensitivity of qualitative, semi-q...
Microbiological diagnosis is crucial for the appropriate management of implant-associated orthopedic infections (IAOIs). Sonication of biomaterials for microbiological diagnosis has not yet been introduced in routine clinical practice. Aim of this study was to describe the advantages and feasibility of this procedure in the clinical setting. We prospectively studied 56 consecutive patients unde...
Background and Aims: Production of acoustic cavitation by sonication has been recently recommended as a targeted treatment. The experimental results from studies indicate that the activity of cavitation generated by bi- or multi-frequency ultrasound irradiation is higher than that caused by single frequency irradiation. In this study, effects dual (1 MHz and 40 kHz) and single frequency soni...
Applications of ultrasound for noninvasive drug and gene delivery have been limited by associated cell death as a result of sonication. In this study, we sought to quantify the distribution of cellular bioeffects caused by low-frequency ultrasound (24 kHz) and test the hypothesis that Ca(2+) chelation after sonication can shift this distribution by saving cells from death by apoptosis. Using fl...
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