Calcium currents (ICa) were measured in frog ventricular myocytes using the whole-cell patch clamp technique and a perfused pipette. To gain insight into the role of G proteins in the regulation of ICa in intact cells, the effect of internal perfusion with hydrolysis-resistant GTP analogues, guanylyl 5'-imidodiphosphate (GppNHp) or guanosine 5'-thiotriphosphate (GTP gamma S), on ICa stimulated ...

BACKGROUND Oxidative stress is involved in the pathophysiology of atherosclerosis, diabetes mellitus, hypertension, obesity, and cigarette smoking, all of these being risk factors for coronary heart disease (CHD). We tested the hypothesis that risk factors of CHD are associated with abundant systemic oxidative stress. METHODS AND RESULTS We conducted a case-control study with 93 CHD patients ...

variation in growth, feed utilization and survival was assessed in sturgeon fingerlings of beluga fed different dietary lipid levels. all diets were iso-nitrogenous (protein = 45%) comprising 3.4-3.7 kcal digestible energy g-1. triplicate groups of 35 fingerling beluga with a mean initial wet weight of 2.14 ± 0.07 g (± sd) were fed with four experimental diets containing 6.7, 10.5, 14 and 17% c...

Gravin, an A-kinase anchoring protein, targets protein kinase A (PKA), protein kinase C (PKC), calcineurin and other signaling molecules to the beta2-adrenergic receptor (β2-AR). Gravin mediates desensitization/resensitization of the receptor by facilitating its phosphorylation by PKA and PKC. The role of gravin in β-AR mediated regulation of cardiac function is unclear. The purpose of this stu...

In freshly dispersed guinea pig taenia coli myocytes the activity of the large conductance Ca(2+)-activated K+ channel (maxi-K+ channel) predominates. The open probability (Po) of this channel is increased by micromolar concentrations of the beta-adrenergic agonist isoproterenol (ISO). Low concentrations of cholera toxin (CTX, 1 pM) and guanosine 5'-O-2-thiodiphosphate (GDP beta S, 0.5 mM) supp...

BACKGROUND/AIMS Resveratrol (Res), a polyphenol antioxidant found in red wine, has been shown to play a cardioprotective role. This study was undertaken to investigate whether Res can protect the heart suffering from hypertrophy injuries induced by isoproterenol (ISO), and whether the protective effect is mediated by endoplasmic reticulum (ER) stress. METHODS Cardiomyocytes were randomly assi...

Isoproterenol (ISO) has been reported to inhibit high mobility group box 1 (HMGB1) protein release via heme oxygenase-1 (HO-1) induction in lipopolysaccharide (LPS)-activated RAW 264.7 cells and increase the survival rate of cecal ligation and puncture (CLP)-induced septic mice. Therefore, it was examined whether ISO-mediated HO-1 induction inhibits HMGB1 release in cardiac myocytes and attenua...

Background: Hospital standards are one of the valuest concepture items in an organization and hospital management. The research purpose was determination of luchs of hospital evaluation current way throght the comparission with ISO quality management standards and suggestion of a model to hospital standard development based on aquired findings. Materials & Methods: This research is a descripti...

β-Adrenergic agonists have been reported to increase lung liquid clearance by stimulating active Na+ transport across the alveolar epithelium. We studied mechanisms by which β-adrenergic isoproterenol (Iso) increases lung liquid clearance in isolated perfused fluid-filled rat lungs. Iso perfused through the pulmonary circulation at concentrations of 10-4 to 10-8 M increased lung liquid clearanc...

Nowadays, textile processing based on biotechnology has gained its importance in the view of stringent environmental and industrial safety conditions. The use of protease enzymes in protein fibers to improve some physical and mechanical properties is particularly interesting. In this research, wool yarns were first treated with different concentrations of protease enzymes in aqueous solution in...