In rabbit skeletal muscle the polycation-stimulated (PCS) protein phosphatases [Merlevede (1985) Adv. Protein Phosphatases 1, 1-18] are the only phosphatases displaying significant activity toward the deinhibitor protein. Among them, the PCSH protein phosphatase represents more than 80% of the measurable deinhibitor phosphatase activity associated with the PCS phosphatases. The deinhibitor phos...

Predictions of protein structure are best tested without prior knowledge of the protein three-dimensional structure. Three-dimensional atomic models will soon be determined by X-ray crystallography for the alpha-subunit of human blood clotting factor XIII and members of the family of protein tyrosine specific phosphatases. Accordingly, we here present secondary structure predictions for each of...

protein tyrosine phosphatases (ptpases) regulate tyrosine phosphorylation of target proteins involved in several aspects of cellular functions. enzyme activities of the ptpases in cytosolic and particulate fractions of rat ascites hepatoma cell lines were determined and compared with those of normal rat liver. our present data revealed that although there was no neoplatic-specific alteration of...

The prevalence of diabetes is increasing rapidly worldwide. A cardinal feature of most forms of diabetes is the lack of insulin-producing capability, due to the loss of insulin-producing β-cells, impaired glucose-sensitive insulin secretion from the β-cell, or a combination thereof, the reasons for which largely remain elusive. Reversible phosphorylation is an important and versatile mechanism ...

A protein phosphatase, designated Pph3, from Myxococcus xanthus showed the enzymatic characteristics of PP2C-type serine/threonine protein phosphatases, which are metal ion-dependent, okadaic acid-insensitive protein phosphatases. The pph3 mutant under starvation conditions formed immature fruiting bodies and reduced sporulation.

According to a modest estimation, one third of the known eukaryotic proteins can be phosphorylated. Protein kinases are members of a large superfamily and incorporate the phosphate moiety into the serine (Ser), threonine (Thr) or tyrosine (Tyr) residues of their target proteins. In comparison, protein phosphatases are less numerous but more diverse. They can be classified into four independent ...

Phosphorylation and dephosphorylation of cellular proteins by protein kinases and phosphatases represent important mechanisms for controlling major biological events. In the nervous system, protein phosphatases are contained in highly dynamic complexes localized within specialized subcellular compartments and they ensure timely dephosphorylation of multiple neuronal phosphoproteins. This modula...

The potential use of microorganisms in the bioremediation U pollution has been extensively described. However, a lack knowledge on molecular resistance mechanisms become challenge for these technologies. We reported transcriptomic and microscopic response Stenotrophomonas bentonitica BII-R7 exposed to 100 250 ?M U. Results showed that exposure displayed up-regulation 185 148 genes during lag ex...