Microbial cells are reversibly associated with surfaces in the form of biofilms. Adhesion is mechanism used by microorganisms to bind a surface initially; no biofilm formed without initial adhesion. The aim this work was evaluate efficacy rhamnolipids Pseudomonas aeruginosa Rn19a inhibiting biofilms clinical isolates Escherichia coli I5, E26, Enterococcus faecalis I27 on borosilicate coupons in...

Influence of the frequently used carbon sources in nitrate removal processes were evaluated in a lab-scale biofilm reactor. The NO3-N removal efficiency was in the order acetate>glucose>methanol>ethanol. Acetate-fed biofilm reduced nearly 100% NO3-N with negligible amount of NO2-N accumulation. Although 99% NO3-N was reduced in the glucose-fed biofilm, substantial NH3-N and NO2-N accumulated. M...

Extracellular DNA is an important component of the biofilm matrix. Now, Pseudomonas aeruginosa is shown to control autolysis through the production of HQNO, a quorum-sensing-regulated respiratory poison. Thus, HQNO-driven autolysis links programmed cell death with quorum sensing and biofilm formation.

Pseudomonas aeruginosa biofilm was cultivated and characterized in a microfluidic "lab-on-a-chip" platform coupled with confocal Raman microscopy in a non-destructive manner. Biofilm formation could be quantified by this label-free platform and correlated well with confocal laser scanning microscopy. This Raman-microfluidic platform could also discriminate biofilms at different developmental st...

Pyocyanin production by Pseudomonas aeruginosa can be monitored using hyperspectral imaging and square wave voltammetry, with electrochemical impedance spectroscopy offering new avenues for low-cost sensing approaches to biofilm characterisation.

Background/aim: Because biofilms are resistant to antibiotics and biocides, they usually cause chronic persistent infections, which are arduous to cure and have high mortality and morbidity. Our study aimed to investigate the efficiency of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite on Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and live m...

The leading cause of morbidity and mortality in cystic fibrosis (CF) continues to be lung infections with Pseudomonas aeruginosa biofilms. Co-colonization of the lungs with P aeruginosa and Burkholderia cepacia can result in more severe pulmonary disease than P. aeruginosa alone. The interactions between P. aeruginosa biofilms and B. cepacia are not yet understood; one possible association bein...

Quorum sensing (QS) is a key regulator of virulence factors and biofilm formation in Gram-negative bacteria such as Pseudomonas aeruginosa. Microorganisms that inhabit soil are of strategic importance in the discovery of compounds with anti-QS properties. The objective of the study was to test the culture extract of a taxonomically novel species of Paenibacillus strain 139SI for its inhibitory ...

The antibacterial and anti-biofilm activity of ethanolic extract from the rhizome of Zingiber officinale were evaluated. In vitro antibacterial activity was investigated by microdilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. The values were in the range from 0.0024 to > 20 mg/ml. The most sensitive bacteria were Gram-po...

background and objectives: there is increasing emergence of multidrug resistant pseudomonas aeruginosa (mdrpa) strains and drug resistance is positively-correlated with biofilm-forming ability. since about 10% of p. aeruginosa genome is controlled by quorum sensing (qs), alteration in its antibiotic susceptibility by targeting qs was the focus of the present study. materials and methods : one d...