Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...

objective: in-time diagnosis of streptococcus pneumoniae (s. pneumonia) can play a significant role in decreasing morbidity and mortality rate. applying molecular methods has gained popularity due to the existing limits of routine diagnostic methods. examining the expression of different genes of this bacterium through different molecular methods suggests that lyta gene has a higher sensitivity...

background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...