Background: Infections due to Pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. At present, aggressive antibiotic therapy is the only choice for management of P. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alt...

background: in the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. signal peptides play a critical role in prosperous secretion of recombinant proteins. accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-er...

objective: escherichia coli o157:h7 is a gram-negative rod-shaped bacterium. e coli o157:h7 is an enterohemorrhagic (ehec) strain of the bacterium escherichia coli and a cause of foodborne illness. infection often leads to bloody diarrhea by producing a toxin called shiga toxin, which damages the intestines, and occasionally leads to kidney failure, especially in young children and elderly peop...

background: interleukin-27 is a heterodimeric cytokine belonging to il-12 and il-23 families, secreted  by antigen presenting cells( apcs ) .the il-27 is composed of 2 subunits: epstein-barr virus induced gene 3 (ebi3) and p28. il-27 is an anti-inflammatory cytokine which has an inhibitory effect on th17 population and suppress the il-17 expression. it is suggested that il-27 could be a potent ...

white spot syndrome virus (wssv) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including iran. in the present study, a pair of primers was designed according to the sequence of vp 28 gene of wssv in the genbank. vp28 gene from an iranian wssv isolate (irvp28) was cloned, sequenced and expressed in escherichia coli bl21(de3) strain in order to produce vp28 protein...

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

The importance of rainbow trout (Onchorhynchus mykiss) in Iran aquaculture industry on one hand, and increasing the mortality of this fish due to outbreaks of infectious diseases, on the other hand, indicate the requirement for more profound understanding the rainbow trout immune system and access to laboratory tools for definitive diagnosis of its diseases. One of the most important defense me...

conclusion: the newly produced recombinant protein bmk revealed to be immunogenic. moreover, anti-bmk antibodies produced in mice were able to recognize both the recombinant bmk neurotoxin and the one in m. eupeus crude venome. taken together, the molecular characterization and recombinant production of the iranian scorpion m. eupeus venom component can serve as a new probe for further studies ...