Extraction of soybean seed proteins for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry analysis is challenging and inconsistent. In this study, we compared four different protein extraction/solubilization methods-urea, thiourea/urea, phenol, and a modified trichloroacetic acid (TCA)/acetone-to determine their efficacy in separating soybean seed proteins by 2D...

This study aims to identify the types of proteins in malted and dry Bambara groundnut seeds and through a comparative analysis, identify similarities and their known uses. Dry viable bambara seed was stored for five days to malt. The proteins in the dry and malted seed were subsequently extracted in potassium phosphate buffer pH 7.0 and precipitated with saturated ammonium sulphate. MudPit (mul...

Huge unrealized post-genome opportunities remain in the understanding of detailed molecular mechanisms for Alzheimer Disease (AD). In this work, we developed a computational method to rank-order AD-related proteins, based on an initial list of AD-related genes and public human protein interaction data. In this method, we first collected an initial seed list of 65 AD-related genes from the OMIM ...

Most plants synthesise proteins in their organs of reproduction and propagation, such as seeds of gymnosperms and angiosperms. Storage proteins are usually located in two tissues. In dicotyledonous plants they may be located in the diploid cotyledons (exalbuminous}, in the triploid endosperm (albuminous) or, occasionally, in both tissues. In monocotyledonous cereals they are primarily located i...

to better understand seed dormancy breaking of bread wheat (triticum aestivum l.rl4137), we examined protein synthesis in dormant and after-ripened seeds.we used six treatments including dormant seed, dormant seed that were subjected to gibberllic acid (ga3) for 10 hours, dormant seed with one, three and six weeks of after-ripening and finally six weeks after-ripened seed subjected to abscisc a...