The limited resolution of serum proteins achieved with a simplified technique of two-dimensional electrophoresis (Clin. Chim. Acta 103: 51-59, 1980) has been improved by using different ampholyte (Ampholine) mixtures in the first dimension, to obtain relatively shallow pH gradients, and prolonged electrophoresis time in the second dimension. The technique has been further simplified, without ne...

The distribution of 35S-sulfate and 3H-glucosamine in the angular region of the hamster was studied by light and electron microscopic autoradiography following intraperitoneal injection of these compounds to hamsters. Exposed silver grains of 35S-sulfate were concentrated in the trabecular meshwork, sclera, and cornea, and grains of 3H-glucosamine were localized in the trabecular region. The ra...

Properties ofa heat-labile glucose transport system in Thiobacillusferrooxidans strain AP-44 were investigated with iron-grown cells. [14C]glucose was incorporated into ceil fractions, and the cells metabolized [14C]glucose to 14CO2. Amytal, rotenone, cyanide, azide, 2,4-dinitrophenol, and dicyclohexylcarbodiimide strongly inhibited [14C]glucose uptake activity, suggesting the presence of an en...

The distribution of S-sulfate and H-glucosamine in the angular region of the hamster was studied by light and electron microscopic autoradiography following intraperitoneal injection of these compounds to hamsters. Exposed silver grains of S-sulfate were concentrated in the trabecular meshwork, sclera, and cornea, and grains of H-glucosamine were localized in the trabecular region. The radioact...

The Iron Mountain Mine Superfund Site near Redding, California, is a massive sulfide ore deposit that was mined for iron, silver, gold, copper, zinc, and pyrite intermittently for nearly 100 years. As a result, both water and air reached the sulfide deposits deep within the mountain, producing acid mine drainage consisting of sulfuric acid and heavy metals from the ore. Particularly, the draina...

The sulfate uptake and accumulation in mouse digestive organs were studied by light microscopic radioautography. Two litters of normal ddY mice 30 days after birth, each consisting of 3 animals, were studied. One litter of animals were sacrificed 30 min after the intraperitoneal injections with phosphate buffered Na2(35)SO4, and the other litter animals were sacrificed 12 hr after the injection...

A chitinase was purified from the stomach of a fish, the silver croaker Pennahia argentatus, by ammonium sulfate fractionation and column chromatography using Chitopearl Basic BL-03, CM-Toyopearl 650S, and Butyl-Toyopearl 650S. The molecular mass and isoelectric point were estimated at 42 kDa and 6.7, respectively. The N-terminal amino acid sequence showed a high level of homology with family 1...

The covalently bound heme moiety in horse heart cytochrome c was cleaved from the polypeptide chain by reaction with silver sulfate in acidic solution. The apoprotein was extracted with acid acetone, treated with 2-mercaptoethanol, and subjected to differential sedimentation to remove the heme moiety, protein bound silver, and polymerized apoprotein, respectively. Monomeric apocytochrome c has ...

Materials. Ethylbromoacetate (97%), potassium carbonate (99%), diethyl ether (99.8%), ethylenediamine (99%), dichloromethane (99.6%), sodium triacetoxyborohydride (97%), sodium borohydride (98%), propionaldehyde (97%), poly(ethylene glycol) methyl ether thiol (PEG-SH, average mw 2000), formic acid (96%), formaldehyde (37% wt in H2O), sodium hydroxide(97%), propionaldehyde (97%), trifluoroacetic...