نتایج جستجو برای: sscp بز مهابادی
تعداد نتایج: 3340 فیلتر نتایج به سال:
هدف از مطالعه حاضر ارزیابی تأثیر آگونیست هورمون gnrh (گنادورلین) بر روی دینامیسم رشد فولیکول¬های تخمدان طی فاز لوتئال در فصل تولیدمثل در بزهای بومی مهابادی بود. 6 رأس بز ماده پس از همزمان¬سازی سیکل استروس در قالب شش گروه یک رأسی قرار گرفته و مطالعه به صورت چرخشی و طی سه سیکل متوالی انجام گرفت. در هر رأس بز، یک سیکل به عنوان کنترل (بدون درمان)، یک سیکل به عنوان gnrh 10 (تزریق 70 میکروگرم گنادورل...
PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic variants. We have adapted this technique for the identification of bacteria by PCR with fluorescein-labeled primers chosen from the conserved regions of the 16S rRNA gene flanking a variable region. The PCR product was denatured, separated on a nondenatu...
Characterization of microbial communities using single-strand conformation polymorphism (SSCP) was compared with that using denaturing gradient gel electrophoresis (DGGE). This comparison was based on the V3-4 region (Escherichia coli positions: 341-806) of 16S rRNA gene of bacterial or archaeal communities obtained from a methanogenic bioreactor. Significant differences in the bacterial bandin...
The recent achievement of the human genome project has led to the identification of many disease genes in common hereditary conditions, in which patients and their relatives would benefit from genetic diagnosis. This has increased the need for simple, sensitive, and cost effective methods of mutation analysis. However, the “gold standard” of mutation analysis, direct sequencing, is still an exp...
To optimize the labeling and visualization of PCR products we tested different variables, including deoxynucleotide concentration and ratio, dilution of labeled product, number of PCR cycles, and use of one-step or nested labeling protocols. Labeling was achieved using a fixed amount of labeled dATP, whose relative specific activity was varied by adding increasing amounts of cold dATP. Optimal ...
The method to internally label PCR products with multiple colored fluorescent dyes was developed and applied to multiple fluorescence-based PCR single-stranded conformational polymorphism (MF-PCR-SSCP) analysis. PCR-amplified fluorescent DNA fragments, which were internally labeled by adding fluorescent dUTPs ([F]dUTPs) to the PCR mixture, were heat-denatured and applied to a nondenaturing poly...
Single-strand conformation polymorphism (SSCP) is a simple method for detecting the presence of mutations in a segment of DNA, but the fraction of all mutations detected is unclear. We have evaluated SSCP for the detection of single-base mutations in the factor IX gene. Multiple conditions were examined including electrophoresis temperature, electrophoresis buffer concentration, acrylamide to b...
This study aims to characterize different Salmonella enterica subsp molecularly. strains (n=49) were isolated from human gastrointestinal cases in the Tolima region and poultry Santander regions using PCR-RFLP, PCR-ribotyping, PCR-SSCP. The band patterns obtained with each technique analyzed by building dendrograms based on Unweighted Pair Group Method Arithmetic mean (UPGMA) method Dice coeffi...
An algorithm to directly identify multiple mycobacterial species in a sample by using fluorescence capillary electrophoresis (FCE)-based single-strand conformation polymorphism (SSCP) analysis was developed. Part of the 16S-23S ribosomal DNA internal transcribed spacer (ITS) region in 37 reference strains and 73 clinical isolates representing 19 mycobacterial species and Mycobacterium tuberculo...
بهمنظور توصیف منحنی تغییرات صفات تولید شیر و وزن بدن در طی دورۀ شیردهی بزهای نژاد مهابادی، مدلهای آماری گوناگون برازش شدند. بدین منظور از 1665 رکورد درخصوص صفات تولید شیر، درصد چربی، مقدار چربی، درصد پروتئین، مقدار پروتئین، درصد لاکتوز، و SNF و 833 رکورد مربوط به وزن بدن که بهصورت روزانه از 123 بز در طی سالهای 1391-1392 جمعآوری شده بودند، استفاده شد. بهمنظور انتخاب بهترین مدل از معیارها...
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