نتایج جستجو برای: using rnease mini kit

تعداد نتایج: 3426840  

Journal: :Methods 2013
Alexandra Kalmar Barnabás Wichmann Orsolya Galamb Sándor Spisák Kinga Tóth Katalin Leiszter Zsolt Tulassay Béla Molnár

Although RNA isolation is a routine process in gene expression analysis studies, the applicability of most widely available formalin-fixed, paraffin-embedded (FFPE) samples is still limited compared to fresh frozen tissue samples due to the lower quality of the isolated RNA. Recently, novel automated isolation methods were developed in order to reduce manual sample handling and increase RNA qua...

Journal: :Japanese journal of infectious diseases 2014
Jun Kawase Morito Kurosaki Yuta Kawakami Takashi Kashimoto Yoshie Tsunomori Koji Sato Tetsuya Ikeda Keiji Yamaguchi Masanori Watahiki Tomoko Shima Mitsuhiro Kameyama Yoshiki Etoh Kazumi Horikawa Hiroshi Fukushima Ryoichi Goto Komei Shirabe

In this study, 2 methods of DNA extraction were evaluated for use in conjunction with the screening system Rapid Foodborne Bacterial Screening 24 (RFBS24), which employs multiplex real-time SYBR Green polymerase chain reaction (SG-PCR) and can simultaneously detect 24 target genes of foodborne pathogens in fecal DNA samples. The QIAamp DNA Stool mini kit (Qkit) and Ultra Clean Fecal DNA Isolati...

Journal: :Molecular and cellular probes 2007
Chris A Whitehouse Hannah E Hottel

Francisella tularensis is the etiologic agent of the zoonotic disease tularemia and is thought to be maintained in the environment principally by various terrestrial and aquatic vertebrate animals. The organism is known to persist in water or mud for long periods of time and Francisella-specific DNA has been identified from water and soil. To gain a better understanding of the ecology and epide...

2012
Sabrina Weiss Kathrin Nowak Jakob Fahr Gudrun Wibbelt Jean-Vivien Mombouli Henri-Joseph Parra Nathan D. Wolfe Bradley S. Schneider Fabian H. Leendertz

Animals obtained for this study were euthanized under anesthesia with xylazine and ketamine by cardiac exsanguination. Tissue samples were extracted by using the NucleoSpin RNA II Kit (Macherey-Nagel, Düren, Germany); throat swab specimens were dissolved in 500 μL of phosphate-buffered saline and extracted by using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany). The same kit was utiliz...

2014
Brian Brestovac Michelle E Wong Paul S Costantino David Groth

method suitable for human papillomavirus detection. Abstract: 33 Infection with oncogenic human papillomavirus (HPV) genotypes is necessary for the 34 development of cervical cancer. Testing for HPV DNA from liquid based cervical 35 samples can be used as an adjunct to traditional cytological screening. In addition 36 there are ongoing viral load, genotyping and prevalence studies. Therefore, a...

Journal: :journal of agricultural science and technology 2010
a. keyhani

to measure soil water content (or soil matric suction) in thin layers of about 30mm, conventional gypsum blocks are not suitable. to carry out the task, mini-gypsum blocks were constructed using plaster of paris in an innovative fashion. a power relationship was found between the soil water content and the mini-gypsum blocks’ readings in kω. the calibration results showed that readings of m...

Introduction: The Mini-Clinical Evaluation Exercise (Mini-CEX ) is widely used to assess clinical performance because of its practicality. Although initial studies have supported its reliability and validity, the utility of Mini-CEX is under serious debate due to inconsistencies in rater judgments. This study aimed to review the relevant literature on the utility of Mini-CEX in order to find ou...

Journal: :Journal of food protection 2006
Saskia A Rutjes Froukje Lodder-Verschoor Wim H M van der Poel Yvonne T H P van Duijnhoven Ana Maria de Roda Husman

Disease outbreaks in which foods are epidemiologically implicated as the common source are frequently reported. Noroviruses and enteric hepatitis A viruses are among the most prevalent causative agents of foodborne diseases. However, the detection of these viruses in foods other than shellfish is often time-consuming and unsuccessful. In this study, three virus concentration methods were compar...

Journal: :The Southeast Asian journal of tropical medicine and public health 2006
T Lier G S Simonsen H Haaheim S O Hjelmevoll B J Vennervald M V Johansen

Chemotherapy has been used on a large scale in countries where the blood fluke Schistosoma japonicum is endemic. This has led to a lower intensity of infections and consequently lower diagnostic values of commonly used diagnostic tests like serology and Kato-Katz stool smear. We designed a novel real-time PCR method for detection of S. japonicum in stool samples. Further, we evaluated different...

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