Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan(®) technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This method is widely used to identify global differences between the expression of 100s of miRNAs acros...

The purpose of this article is to critique and reshape one of the fundamental paradigms of Human-Computer Interaction: the workspace. This treatise argues that the concept of a workspace— as an interaction metaphor— has certain intrinsic defects. As an alternative, a new interaction model, the communication space is offered in the hope that it will bring user interfaces closer to the ideal of h...

To the Editor: The re-analysis of the INSIGHT trial presented by Mancia and associates recently in Hypertension1 is questionable in some aspects and may not have internal validity. Any conclusion based on analysis of subgroups with a small proportion of the participants of the entire trial should be taken as hypothesis generating and may invalidate it if not established a priori.2 This report d...

The perception of prosodic structure (phrasal prominences and boundaries) may depend in part on acoustic cues in the speech signal and in part on utterance meaning as related to syntactic structure and discourse context. In this study we ask if listeners are able to differentially weigh acoustic and meaningbased cues to prosody. We test naïve subjects’ transcription of prominences and boundarie...

We describe a novel biphasic regulation of Il2 transcription in naive CD4(+) T cells. Few ( approximately 5%) CD4(+) T cells transcribe Il2 within 6 h of anti-TCR-beta plus anti-CD28 stimulation (early phase). Most naive CD4(+) T cells do not initiate Il2 transcription until after an additional approximately 12 h of T cell stimulation (late phase). In comparison, essentially all previously acti...