The increase in proline induced by ABA, a process stimulated by NaCl or KCl in barley leaves, did not occur when Na(+) (or K(+)) was present in the external medium as the gluconate salt, namely with an anion unable to permeate the plasma membrane. However, proline increase was restored, to different extents, by the addition of various chloride salts but not by ammonium chloride. Moreover, it wa...

The membrane carrier for L-proline (product of the putP gene) of Escherichia coli K12 was solubilized and functionally reconstituted with E. coli phospholipid by the cholate dilution method. The counterflow activity of the reconstituted system was studied by preloading the proteoliposomes with either L-proline or the proline analogues: L-azetidine-2-carboxylate or 3,4-dehydro-L-proline. The dil...

silicon is known to ameliorate the deleterious effects of drought on plant growth. we evaluated growth of wheat (triticum aestivum l. cv. chamran) under water-deficit stress induced by polyethylene glycol as affected by si application. in this article, the effects of si (as potassium silicate) on some parameters related to growth, chlorophyll concentration relative water content (rwc), electrol...

Since nothing appeared to be known as to the fate of proline in the animal body, it seemed desirable to make some experiments in the hope that some light might be thrown upon the changes which this amino-acid may undergo. As a first step in the investigation, observations were made on the behavior of proline when added to blood used for perfusing a surviving dog’s liver, using essentially the m...

We previously isolated a mutant which showed a high tolerance to freezing that correlated with higher levels of intracellular L-proline derived from L-proline analogue-resistant mutants. The mutation responsible for the analogue resistance and L-proline accumulation was a single nuclear dominant mutation. By introducing the mutant-derived genomic library into a non-L-proline-utilizing strain, t...

Ketoprofen is a non-steroidal anti-inflammatory compound (NSAID) which has low solubility in water. The aim of this study was to increase the dissolution rate ketoprofen by forming multicomponent ketoprofen-Proline with ratio 1:1, 1:2, and 2:1 using solid state grinding method. Samples were characterized Powder X-Ray Diffractometry (PXRD), Differential Scanning Calorimetry (DSC), Fourier Transf...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

Proline accumulation in Escherichia coli is mediated by three proline porters. Proline catabolism is effected by proline porter I (PPI) and proline/delta 1-pyrroline carboxylate dehydrogenase. Proline did not accumulate cytoplasmically when E. coli was subjected to osmotic stress in minimal salts medium. Although PPI is induced when proline is provided as carbon or nitrogen source, its activity...

The effect of wilting on proline synthesis, proline oxidation, and protein synthesis-all of which contribute to proline accumulation-was determined in nonstarved barley (Hordeum vulgare L.) leaves. Nonstarved leaves were from plants previously in the light for 24 hours and starved leaves were from plants previously in the dark for 48 hours. Wilted leaves from nonstarved plants accumulated proli...