نتایج جستجو برای: yeast expression vector

تعداد نتایج: 1122471  

Journal: :modares journal of medical sciences: pathobiology 2010
mehdi mahdavi massoumeh ebtekar kayhan azadmanesh fereidoun mahboudi hamidreza khorram khorshid

objective: today, aids is considered as a global problem and many efforts to generate an effective vaccine against this disease have been made, but remain inconclusive. dna vaccines are a member of the new generation of vaccines that can efficiently stimulate the immune system. however, recent findings indicate low immunogenicity for these vaccines and it is believed that these types of vaccine...

F Sabahi , M Ravanshad , M Saberi-Firoozi , MH Modarressi , SM Moazzeni , SY Hosseini ,

Background and Aims: DNA constructs containing HCV antigens have become one of the vaccine candidates for induction of anti-HCV cellular and humoral immunity. In this study, we constructed a novel expressing vector harboring a fusion sequence derived from an overlapping fragment in the middle of NS3 and a truncated core fragment to avoid troubles reported to be associated with full gene express...

ژورنال: طب جنوب 2023
Farshadpour , Fatemeh, Naeimi , Sirous, Rahimpour , Azam, Rahimpour , Maryam, Taherkhani , Reza,

Background: Vaccines have played a special role in controlling and reducing mortality from infectious diseases. In this regard, DNA vaccines were developed to ease the production and reduce the risks of traditional vaccines. Human papillomavirus (HPV) has been introduced as the causing agent of cervical cancer. The capsid protein (L1) of HPV has been used to produce subunit and DNA vaccines. Th...

ژورنال: یافته 2014
حبیبی‌رودکنار, مهریار, حلبیان, راحله, شیخیان, علی, محمدی‌پور, مهشید, کاشی, معصومه, کیانی, علی‌اصغر,

Background : Genetic manipulation is an effective strategy to protect cells against environmental damages and enhance their capabilities for therapeutic usage. In order to avoid unwanted side effects, such as cancers, the expression of genes should be temporary increased. The aim of this study was to clone and temporary increased expression of a cell protective gene, Metallothionein 1 (MT1) in ...

Amir Babak Sioofy Khojine, Mohammad Saeid Hejazi,

Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...

Bovine chymosin enzyme is one of the most commonly used enzymes in the dairy industry. The production of this enzyme from its natural source does not meet the needs of this huge industry. The production of recombinant bovine chymosin in plants can be a good alternative to native enzyme. Insertion and expression of foreign genes in plants can occur in the nucleus and chloroplast organelles. The ...

Journal: :iranian biomedical journal 0
محمد سعید حجازی mohammad saeid hejazi امیربابک سیوفی خوجینه amir babak sioofy khojine

knowing that adenoviral vectors could initiate innate immunity, the ability of e1-deleted recombinant adenovirus (ad-e1δ) in induction of b7.1 and il-2 molecules was studied. methods: the expression of green fluorescent protein in c1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...

Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. Therefore, designing new and effective therapeutics is of great importance. The aim of the current study was to construct a DNA vaccine containing structural proteins of HCV and evaluation of its expression in a eukaryot...

آجورلو, مهدی, ایرانی, شیوا, غلامی, علیرضا, فنائی, خدیجه, مژگانی, سید حمیدرضا,

Background: Rabies is an acute encephalitis that causes more than 60,000 deaths worldwide. The only way to save individuals bitten by a rabies-infected animal is the timely use of effective vaccines. Treatment with new generation vaccines is expensive. Therefore, there is a global movement towards the production of less expensive vaccines which retain and improve upon the quality and effectiven...

Journal: :jundishapur journal of microbiology 0
mohammad amin behzadi professor alborzi clinical microbiology research center, namazi hospital, shiraz university of medical sciences, shiraz, ir iran; student research committee, shiraz university of medical sciences, shiraz, ir iran abdolvahab alborzi professor alborzi clinical microbiology research center, namazi hospital, shiraz university of medical sciences, shiraz, ir iran gholamreza pouladfar professor alborzi clinical microbiology research center, namazi hospital, shiraz university of medical sciences, shiraz, ir iran mehdi dianatpour stem cell and transgenic technology research center, shiraz university of medical sciences, shiraz, ir iran; department of medical genetics, school of medicine, shiraz university of medical sciences, shiraz, ir iran mazyar ziyaeyan professor alborzi clinical microbiology research center, namazi hospital, shiraz university of medical sciences, shiraz, ir iran; professor alborzi clinical microbiology research center, namazi hospital, shiraz university of medical sciences, p. o. box: 7193711351, shiraz, ir iran. tel: +98-7136474304, fax: +98-7136474303

conclusions this study can serve as a fundamental experiment for the construction of a ns3/ns4a eukaryotic expression vector and its expression in mammalian cells. further research is underway to evaluate the fragment immunogenicity in lab animal models. results the results showed that the fragment was successfully amplified and cloned into a eukaryotic expression vector. sequencing and enzyme ...

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