To assess the performance of second reading chest compute tomography (CT) examinations by expert radiologists in patients with discordance between reverse transcription real-time fluorescence polymerase chain reaction (RT-PCR) test for COVID-19 viral pneumonia and CT report. Three hundred seventy-eight were included this retrospective study (121 women 257 men; 71 years median age, a range 29–93...

Abstract Severe acute respiratory syndrome coronavirus 2 causing disease 2019 pandemic is an enveloped virus, showing genome similarity with bat coronavirus. This virus initially infects the upper tract, subsequent spread to lower tract. Despite availability of antigen and antibody detection methods, reverse transcription-polymerase chain reaction (RT-PCR) diagnostic test choice for this novel ...

Abstract Background We aimed to investigate the association of initial chest CT scan findings with status and adverse outcomes COVID-19 (including ICU admission, mortality, disease severity). This retrospective cohort study was performed in three hospitals Babol, northern Iran, between February March 2020. Cases were confirmed by real-time polymerase chain reaction (RT-PCR). Clinical paraclinic...

Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes. A total of 146 clinical specimens from 143 patients who showed acute respiratory tract infection symptoms were tested by...

background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...