Although the pathogenesis of ankylosing spondylitis (AS), which is a systemic disease characterized by inflammation, largely unknown, pro-inflammatory mediators, oxidative stress, and immunity are thought to be involved in development prognosis disease. It was aimed this study reveal activities adenosine deaminase (ADA), cornerstone enzyme different pathways. Twenty nine AS patients 16 healthy ...

Three ammonia-producing enzymes were selected for study, namely adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4), AMP deaminase (AMP aminohydrolase, EC 3.5.4.6) and urease (urea amidohydrolase, EC 3.5.1 3). Activities were detected after each enzyme preparation was subjected to electrophoresis on both starch gel and cellulose acetate strips. Adenosine deaminase activity had been detec...

L-arginine is conditionally essetcial amino acid, required for normal cell growth, protein synthesis, ammonia detoxification, tissue growth and general performance, proposed in the treatment of men sterility and prevention of male impotence. The aim of the present paper was to estimate the activity of the enzymes of adenine nucleotide metabolism: 5'-nucleotidase (5'-NU), adenosine deaminase (AD...

1 Valdes L. Diagnosis of tuberculous pleurisy using the biologic parameters adenosine deaminase, lysozyme, and interferon gamma. Chest 1993; 103:458-65 2 Ocaiia I, Martinez-Vazquez JM, Segura RM, Fernandez de Sevilla T, Capdevila JA. Adenosine deaminase in pleural fluids: test for diagnosis of tuberculous pleural effusion. Chest 1983; 84:51-3 3 Van Keimpema ARJ. Adenosine deaminase activity, no...

The effect of physiological concentrations of caffeine (purified from Persian tea) on adenosine deaminase (ADA) activity at physiological and pathological concentrations of adenosine (as substrate) in 50 mM Tris-HCl buffer (pH 7.3) at 37°C was investigated, using UV-VIS spectroscopy. ADA exhibited a bi-phasic activity behavior and both phases showed positive cooperativities indicating adenosine...

Human erythrocyte adenosine deaminase has been purified approximately 800,000-fold to apparent homogeneity using antibody affinity chromatography. The enzyme was shown to be a single polypeptide chain with an estimated molecular weight of approximately 38,000. The three electrophoretic forms of erythrocyte adenosine deaminase purified simultaneoudy by this technique were indistinguishable by so...

Epinephrine, norepinephrine and isoproterenol completely inhibited insulin-stimulated 2-deoxyglucose uptake by rat adipocytes, but only when adenosine was prevented from accumulating in the incubation medium by addition of adenosine deaminase. Basal uptake rates were not affected. The effects were not observed in the presence of adenosine deaminase plus N6-phenylisopropyl adenosine (a non-metab...

The nucleoside antibiotic formycin, 7-amino-3-(beta-D-ribofuranosyl)pyrazolo(4,3-d)pyrimidine, a structural analogue of adenosine, is deaminated about 10-fold faster by adenosine deaminase than adenosine itself, and is therefore a superior substrate for both routine assays and kinetic studies with the purified enzyme. The luminescence properties of formycin have been profited from to develop a ...

Mammalian adenosine deaminase has been shown by genetic and biochemical evidence to be essential for the development of the immune system. For the purpose of studying the function and structure of this enzyme, we have isolated by genetic selection a mouse cell line, B-1/50, in which adenosine deaminase levels were increased 4,300-fold over the parent cell line. The enzyme was purified from thes...