An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, alpha-amylase, and beta-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the trans...

The Inactivation kinetics of alpha-glucosidase, glucoamylase, alpha-amylase, and acid carboxypeptidase in fresh sake using a continuous flow system for high-pressure carbonation were investigated. In addition, the effects of ethanol and sugar concentrations on inactivation of the enzymes in high-pressure carbonated sake were investigated. Among the enzymes investigated, alpha-glucosidase was th...

Effects of chemical and heat pretreatments on the protein, gluten, and alpha-amylase activity, pasting (Peak [P], Final [F] setback [S] viscosity, pasting temperature [PT] and time [T]) and alveogram (Energy [E], maximum inflation [MI], P/L, and elasticity index [EI]) properties of flour from the pretreated bananas and its composite with wheat flour (WF) were examined. The baking (water absorpt...

The phytochemical investigation on Indigofera heterantha root oil resulted in the identification of 121 phytochemicals using GC-MS analysis. These were docked against alpha-amylase, alpha-glucosidase enzymes. docking results suggested that Hexacosyl acetate (121) possess alpha-amylase inhibitory potential with a score ?8.2944994 and interaction enzyme was ?9.73762512, followed by 9, 12, 15-Octa...

introduction: tobacco smoke is involved in the pathogenesis of several diseases in different body systems. saliva is the first body fluid that confront inhaled cigarette smoke which is injurious to the oral cavity and is associated with several oral diseases and cancer. the aim of our study was to evaluate the influence of smoking on the activity of alpha-amylase in the saliva of healthy smoker...

Th e paper presents numerical comparison of two methods of estimation of nonallelic interaction of QTL eff ects. In the fi rst method we assume that we observe only the plant phenotype, while in the second method we have additional information from the molecular markers observations. In this paper we analysed phenotypic data on 120 barley doubled haploid lines, derived from cross Clipper × Saha...

Yorkstar wheat, grown in New York State, has a high percentage (10-11) of grains without embryos. The embryoless grains have viable aleurone layers and show no sign of injury. These grains are able to support alpha-amylase synthesis only in the presence of gibberellin A(3) (GA(3)). In the absence of GA(3) some protein synthesis occurs in embryoless grains during the early hours of soaking, indi...

OBJECTIVE The pathogenesis of autoimmune pancreatitis (AIP) and fulminant type 1 diabetes remains unclear, although it is known that immune-mediated processes severely compromise the endocrine and exocrine functions in both diseases. RESEARCH DESIGN AND METHODS We have screened a lambdaTriplEx2 human pancreas cDNA library with serum from a patient with AIP and obtained positive clones. Sequen...