نتایج جستجو برای: amplified refractory mutation system
تعداد نتایج: 2553865 فیلتر نتایج به سال:
"Adaptive mutation" denotes a collection of processes in which cells respond to growth-limiting environments by producing compensatory mutants that grow well, apparently violating fundamental principles of evolution. In a well-studied model, starvation of stationary-phase lac(-)Escherichia coli cells on lactose medium induces Lac(+)revertants at higher frequencies than predicted by usual mutati...
effects of induced mutation via gamma irradiated with 250 gy dose (in nuclear agriculture research school- nuclear science and technology research institute) on trichoderma harzianum conidiaon chitinase activity by sts molecular marker have been evaluated in this study. among 20 irradiated mutants which selected via improved antagonistic capability against rhizoctonia solani, pcr amplified chit...
PURPOSE To evaluate mutations in the visual system homeobox gene 1 (VSX1) and superoxide dismutase 1 (SOD1) genes with keratoconus (KTCN), direct sequencing was performed in an Iranian population. METHODS One hundred and twelve autosomal dominant KTCN patients and fifty-two unaffected individuals from twenty-six Iranian families, as well as one hundred healthy people as controls were enrolled...
beta thalassemia is the most common autosomal recessive disorder. the present study reports a rare β globin gene mutation, hbb: c.180g>a: codon 59 (aag/aaa), in a patient from gilan province, northern iran. nucleotide sequencing of amplified dna belonging to a 35 years old man presenting mild hypochromia revealed a synonymous mutation due to a g>a conversion at the third position of codon 59 o...
Fig. S1. (a, b) Agarose gel analysis and (c, d) direct sequencing of mRNA amplified by nested PCRs in the proband and her mother. The mutation c.488delG in exon 5 of LAMA3 has been amplified using a forward primer located in exon 1 (bp 175) and a reverse primer located in exon 8 (bp 815). The mutation c.4484C>T in exon 33 of LAMA3 has been amplified using a forward primer located in exon 31 (bp...
Objective(s) P53 is an important tumor suppressor, which is mutated in later stages of many cancers and leads to resistance to chemotherapy. The aim of this study was to reveal mutations of TP53 in colorectal cancer in Kerman province. Materials and Methods A total of Forty-three colon cancer specimens as paraffin block or fresh tissues, which passed stage IIIA, were selected. Three exons 5,...
OBJECTIVE To examine molecular genetics and prenatal diagnosis of beta-thalassaemia. METHODS The study was conducted at the COMSATS Institute of Information Technology, Sahiwal, Pakistan, from October 2012 to October 2013, and comprised families having children affected by thalassaemia and autosomal recessive b-thalassemia. Blood samples of thalassaemic children and their parents were collect...
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