Structure and organization of two complete ribosomal RNA (rRNA) gene sets, rrnO and rrnA, were determined for the first time in Bacillus subtilis. They are located at the region of the replication origin of the chromosome. Each set constitutes a single operon of: two tandem promoters - leader sequence - 16S rRNA gene - Ile-tRNA gene - Ala-tRNA gene - 23S rRNA gene - 5S rRNA gene - termination s...

gbss protein, that is the product of waxy gene (wx) is one of the major enzyme in starch biosynthesis that controls the proportion of amylose to amylopechtine and its absence leads to production of starch with low amylose and higher amylopechtine content, called waxy starch.wx-a1, wx-b1, wx-d1 are three locuses in bread wheat (triticum aestivum) genome that encodes gbss protein and waxy starch ...

Barrier to autointegration factor (BAF/BANF1) is a cellular DNA-binding protein found in the nucleus and cytoplasm. Cytoplasmic BAF binds to foreign DNA and can act as a defense against vaccinia DNA replication. To evade BAF, vaccinia expresses the B1 kinase, which phosphorylates BAF and blocks its ability to bind DNA. Interestingly, B1 is also needed for viral intermediate gene expression via ...

indole acetic acid is an important hormone that improved the growth of different plant organs such as the root under normal and stress conditions. as various studies have shown, most of rhizobacteria produce indole acetic acid and thereby contribute to the growth of plants. in this study, different rhizobacteria were isolated from different regions of iran for the assessment of their ability to...

Abstract Faeces from cows with different milk yield and non-lactating were applied to soil investigate whether N 2 O efflux is related feeding-induced differences in faecal microbiome abundances of nitrification denitrification genes. Fungal 18S-rRNA gene abundance was the highest faeces group. The 18S-rRNA/ergosterol ratio showed a strong positive correlation 18S-rRNA/fungal glucosamine ratio....

background:  aeromonas spp. can cause diarrhea and various infections in humans. access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of aeromonas species. the aim of this study was to evaluate two different methods including api 20e bacterial identification tests and the molecular detection using pcr primers specific for 16s-rrna and 23s-...

The copy numbers of 16S rRNA genes in 12 probiotic Lactobacillus strains of poultry origin were analyzed. Genomic DNA of the strains was digested with restriction endonucleases that do not cut within the 16S rRNA gene of the strains. This was followed by Southern hybridization with a biotinylated probe complementary to the 16S rRNA gene. The copy number of the 16S rRNA gene within a Lactobacill...

The B1 gene of vaccinia virus encodes a serine/threonine protein kinase that is expressed early after infection. Under nonpermissive conditions, temperature-sensitive mutants (ts2 and ts25) that map to B1 fail to efficiently replicate viral DNA. Our goal was to extend studies on the function of B1 by determining if the kinase is required for intermediate or late gene expression, two events that...

The regulation of activity of ribosomal RNA genes has been studied in euploid and aneuploid varieties of hyacinth. The gene/gene product relationship was determined by measuring the specific gene product (stable rRNA) and the total gene product (protein) in varieties with different dosages of rRNA genes. The results show that the cellular rRNA content is positively regulated, that maximum gene ...