نتایج جستجو برای: b3 loci by through specific primers
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Resistance Gene Analog (RGA) strategy is being exploited perfectly for the identification, tagging and mapping of major genes or Quantitative Trait Loci for disease resistance. About 29 RGA primers designed from the conserved domains of resistance proteins, were used to analyse the genetic diversity among the 40 sugarcane cultivars that vary in their resistance to red rot disease. The genetic s...
Microsatellites are popular genetic markers in molecular ecology, genetic mapping and forensics. Unfortunately, despite recent advances, the isolation of de novo polymorphic microsatellite loci often requires expensive and intensive groundwork. Primers developed for a focal species are commonly tested in a related, non-focal species of interest for the amplification of orthologous polymorphic l...
Singh, Baljit, Chenzhong Fu, and Jahar Bhattacharya. Vascular expression of the avb3-integrin in lung and other organs. Am. J. Physiol. Lung Cell. Mol. Physiol. 278: L217–L226, 2000.—The expression of the avb3-integrin in nonproliferating vascular beds remains unclear. To determine possible organ-specific differences, we compared avb3integrin expression in the lung and other organs. Paraffinemb...
Finding association between molecular markers and agronomic traits provides an excellent tool for indirect selection of a trait of interest in the population. In this study, stepwise regression analysis was used to estimate associations between ISSR and RAPD markers with some agronomic traits in 12 lemon balm ecotypes. Markers or bands were considered as independent variables and traits were co...
Abstract A major technical hurdle for T cell immune profiling is the time and cost to accurately genotype Human Leukocyte Antigen (HLA) loci from peripheral blood. Here, we developed a rapid, highly multiplexed approach HLA typing using RNA <100,000 blood mononuclear cells with Oxford Nanopore Technology (ONT) Minion sequencer. This method uses selective reverse transcription of mRNA six...
as some definitions show, idioms are expressions whose meanings cannot be obtained from individual words. in every society, people use their own conceptions and feelings through different idioms and expressions. so every culture and society has their own idioms. some scholars proposed methods for translating idioms but baker’s strategies are very important and constructive. this research tried ...
serology and dna techniques are employed for human leukocyte antigen (hla) typing in different transplant centers. results may not always correlate well and may need retyping with different technique. all the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring hla typing for bone marrow transplant were enrolled in the study. serological hla typing was ...
Background: The aim of this study was compared the efficacy of the designed primers and already published primers for detection of the exoA, oprL and algD genes by PCR assay for finding a rapid, accurate and highly sensitive and specific procedure to detect the Pseudomonas aeruginosa in the serious and fatal infections such as cystic fibrosis disease, burned individual.Methods: A total of 150...
Agropyron cristatum (P genome) is a Triticeae species from the wheat tertiary gene pool which has economic importance as forage and also displays traits beneficial to wheat. Resistance leaf rust was previously mapped short arm of chromosome 1P (1PS) in A. by development compensating Robertsonian translocation involving 1PS long 1B (1BL). In this study, engineered using ph1b mutation induce 1BS/...
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