BACKGROUND Chlorophytum borivilianum is an industrially valued medicinal crop. Propagation through seeds is not feasible because of low germination percentage and long dormancy period. Therefore, callus culture and plant regeneration can be an alternative to improve this crop production. Also, callus can serve as an alternative source of bioactive compounds. OBJECTIVE To evaluate the effect o...

Vitamins are necessary compounds synthesized and utilized in plants. In tissue culture media, vitamin addition is not always common; since the amount needed by plants is relatively unknown and varies. Vitamins, in combination with other media constituents, have been shown to have direct and indirect effects on callus growth, somatic growth, rooting, and embryonic development. For example, diffe...

BACKGROUND Chonemorpha grandiflora (Syn. Chonemorpha fragrans (Apocynaceae) is an endangered medicinal plant. It is used in different preparations, such as sudarsanasavam and kumaryasavam used in Kerala Ayurvedic system. C. grandiflora is used for the treatment of fever and stomach disorders. Phytochemical investigations have revealed the presence of steroidal alkaloids, such as chonemorphine a...

Elicitation effect on the production of asiaticoside and asiatic acid in shoot, callus, cell suspension culture Centella asiaticaM. Laxmi Krishnan, Arpita Roy, Navneeta Bharadvaja

Glyphosate (N-phosphonomethylglycine) caused a significant decrease or a slight increase in ethylene production in tobacco callus (Nicotiana tabacum L.) depending on the concentration of indole-3-acetic acid (IAA) present in the medium. IAA stimulated ethylene production, but a pretreatment with glyphosate greatly reduced the IAA-induced ethylene production. Inasmuch as glyphosate treatment pro...

pseudohandelia tzvel. belongs to the asteraceae family with only one species. it is endemic to khorasan province in iran. having partially big and beautiful inflorescences make it a proper choice in urban green space especially in case of mass culture. in this research, to investigate possibility of micro propagation of this plant two separate experiments were designed. to obtain non contaminat...

We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no esca...

In five cultivated rice varieties e.g., Nonabokra, PNR 381, Taipei 309, Koshika and TKM 11 the response in the initiation of callus and its size varied greatly among varieties, salt concentrations (NaCl and Na2SO4) and their interaction. Better regeneration ability of Nonabokra, Koshika and TKM 11 was found to be correlated to small size callus with a high concentration of Na2SO4. The regenerat...

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 we...