The catalysis of the energy-linked transhydrogenation reaction of Rhodospirillum rubrum chromatophores is dependent upon a soluble protein, the transhydrogenase factor. This factor can be detached from the chromatophore membrane by the relatively mild procedures of extensive washing, passage of chromatophores through Sephadex G-ZOO, or sedimentation of chromatophores through a dense sucrose sol...

The mitochondrial DNA polymerase from Crithidia fasciculata has been purified to near homogeneity. SDS-PAGE analysis of the purified enzyme reveals a single polypeptide with a molecular weight of approximately 43,000. The protein is basic, with an isoelec; tric point between 7.6-8.0. Its Stokes radius of 22 A and its sedimentation coefficient of 4.1 S suggest a native molecular weight of 38,000...

Adeno-associated virus (AAV) replicates its DNA exclusively by a leading-strand DNA replication mechanism and requires coinfection with a helper virus, such as adenovirus, to achieve a productive infection. In previous work, we described an in vitro AAV replication assay that required the AAV terminal repeats (the origins for DNA replication), the AAV Rep protein (the origin binding protein), a...

Supercritical carbon dioxide (SC-CO,). fractionation technique was evaluated as an altemativeprocess for reducing the free-fatty-acid (FFA) content and minimizing the phytosterolloss of rice bran oil (REO) during the process. The effects of pressure (20.5 to 32.0 MPa) and temperature (45 to 80 DC) for isothermal operation of the column on the composition of the resultant fractions were exarnine...

BACKGROUND Coula edulis Bail (Olacaceae), is an evergreen tree growing to a height of 25. This study aimed at evaluating the antidermatophytic and toxicological properties of the stem bark of C. edulis extract as well as fractions and compounds isolated from it. METHODS The plant extract was prepared by maceration in CH(2)Cl(2)-MeOH (1:1 v/v). The fractionation of this extract was done by sil...

Arthrobacter sp. 10137 has been used to produce b-fructofuranosidase (FFase). Sucrose and corn steep powder in an optimized ratio of 10:1 were the best carbon and nitrogen sources for enzyme production in a shake flask. The maximum FFase activity was 26.69 U/mL after 22.5 h in batch culture, and the crude FFase, obtained by ultrafiltration and (NH4)2SO4 fractionation, was purified about 7-fold ...

Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of pronase digestion (Step 1), cetylpyridinium chloride-fractionation (Step 2), crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment (Step 3) and preparative cellulose acetate membrane-electrophoresis (Step 4). By this method, sulfated glycopeptide was obtained in ...