PURPOSE To compare polymerase chain reaction (PCR) to microbial culture for the detection and identification of bacterial and fungal pathogens in microbial keratitis. DESIGN Prospective cohort study. METHODS A total of 108 consecutive corneal ulcers were cultured and analyzed by PCR using pan-bacterial and pan-fungal primers. PCR products were cloned, sequenced, and compared to culture resu...

background and objectives: the aim of this study was to compare direct microscopic examination with culture and pcr for the diagnosis of mucorales infection in blood and tissue specimens. material and methods: blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with suspected invasive fungal infection and cultured on proper media. direct smear of tissue samples ...

Rapid and accurate identification of mastitis pathogens is important for disease control. Bacterial culture and isolate identification is considered the gold-standard in mastitis diagnosis but is time-consuming and results in many culture-negative samples. Identification of mastitis pathogens by PCR has been proposed as a fast and sensitive alternative to bacterial culture. The results of bacte...

AIMS The purpose of this study was to determine the clinical significance of detecting microbial footprints of ureaplasmas in amniotic fluid (AF) using specific primers for the polymerase chain reaction (PCR) in patients presenting with cervical insufficiency. METHODS Amniocentesis was performed in 58 patients with acute cervical insufficiency (cervical dilatation, > or =1.5 cm) and intact me...

background and objective: due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission. materials and methods: to detect b. pertussis strains, we used two real-time pcr targeting is 481 and bp283 sequences and compared factors influencing culture and real-time pcr results. results: totally, 779 specimens were...

mycobacterium avium subspecies paratuberculosis (map) is the cause of john’s disease also called paratuberculosis. this is economically one of the important infectious diseases in cattle and ruminant husbandry. this disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. the typical sign of this disease is progressive loss of weight. cons...

Ghar-e-Dagh (Nitraria schoberi) is a native perennial plant profoundly resistant to salinity and drought. This plant, having a well-developed deep rooting system can act as sand dunes stabilization agent and also, due to its special alkaloids may perform a very significant role in pharmaceutical industries. Considering the importance of genetic engineering as a novel method to generate...

Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...