Cassava brown streak disease (CBSD), caused by Cassava brown streak virus (CBSV), is an economically important disease of cassava (Manihot esculenta Crantz) in East Africa. The objective of this study was to optimise in vitro techniques for CBSV elimination from infected Ugandan cassava cultivars. Using semi-solid halfstrength Murashige and Skoog (MS) basal medium, hormone concentration and hea...

The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA a...

An in vitro micropropagation protocol was developed for Capsicum chinense Jacq. popularly known as habenaro pepper which is one of the world’s hottest chilli and it is a traditional food item of the Naga people. Nagaland is believed to be the original home for the chilli pepper. The Guinness book of World record has awarded this chilli as hottest one as it contains 1,041,427 Scoville Heat units...

Therapeutic hypothermia (TH) is thought to be due to the downregulation of free radical production, although the details of this process remain unclear. Here, we investigate changes in oxidative stress and endogenous biological antioxidant potential during TH in patients with post-cardiac arrest syndrome (PCAS). Nineteen PCAS patients were enrolled in the study. Brain temperature was decreased ...

Knowledge of the ability of the female reproductive system to metabolize environmental chemicals is critical not only from the standpoint of toxicity but also from infertility risk assessment. Benzo(a)pyrene (BaP) is a toxicant that is released into the environment from automobile exhausts, cigarette smoke, burning of refuse, industrial emissions, and hazardous waste sites. In exposed animals, ...

Benzo[a]pyrene (BaP) is a human carcinogen that covalently binds to DNA after activation by cytochrome P450 (P450). Here, we investigated whether NADH:cytochrome b5 reductase (CBR) in the presence of cytochrome b5 can act as sole electron donor to human P450 1A1 during BaP oxidation and replace the canonical NADPH:cytochrome P450 reductase (POR) system. We also studied the efficiencies of the c...

Rice plant regeneration via anther culture possess several difficulties, these included early necrosis and high albinism frequency. In the present study, biotic abiotic factors were studied to develop an efficient protocol for of Malaysian indica rice MR 219 variety. Callus initiation cultures was evaluated using different N6 media supplemented with 2,4-D in combination 1-naphthaleneacetic acid...