objective: today, aids is considered as a global problem and many efforts to generate an effective vaccine against this disease have been made, but remain inconclusive. dna vaccines are a member of the new generation of vaccines that can efficiently stimulate the immune system. however, recent findings indicate low immunogenicity for these vaccines and it is believed that these types of vaccine...

The present study was to construct a parentage verification system for Iranian Caspian horse. A total number of 45 Caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. Genomic DNA was extracted from whole blood and the genotype were analysed by PCR procedure and using 7 microsatellite markers (AHT04, HMS03, HM...

Various extensions of public announcement logic have been proposed with quantification over announcements. The best-known extension is called arbitrary public announcement logic, APAL. It contains a primitive language construct ✷φ intuitively expressing that “after every public announcement of a formula, formula φ is true.” The logic APAL is undecidable and it has an infinitary axiomatization. ...

A recently developed active electronic personal dosemeter (AEPD) was utilised in order to measure the levels and the structure of occupational exposure to scattered X-ray radiation of medical staff who performed percutaneous revascularisation therapy that involves interventional radiology (IR) on the pelvis and upper leg arteries. The AEPDs, placed on the operators' and assistants' chests, that...

I study dynamic games where players may be unaware of some fundamentals of the game itself, such as actions or players, and such unawareness may be lifted by other players’ actions during the play. Based on Li (2006a, 2006b), I propose a framework to deal with such games using standard tools of game theory. I construct examples to show that unawareness is distinctly different from assigning zer...

Humble leadership behavior consists of acknowledging personal faults, mistakes and limits; spotlighting their followers’ contributions and strengths; modeling teachability. Previous studies have suggested that humble leadership behavior leads to a number of positive outcomes for organizations, such as high levels of learning goal orientation, performance and engagement, yet almost nothing has b...

there are many techniques to knock out directed genes in bacteria, some of which have been described in salmonella species. in this study, a combination of soeing pcr method and the λ red disruption system were used to disrupt phop gene in wild type and standard strains of salmonella typhimurium. three standards pcr and one fusion pcr reactions were performed to construct a linear dna including...

Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs. siRNA is recognized and processed by Ago2 and C3PO, endonucleases of the RNA-induced silencing complex (RISC). RISC cleaves passenger RNA, exposing the guide RNA for base-pairing with its homologous mRNA target. Remarkably, the ...

The design and generation of an optimal expression construct is the first and essential step in in the characterization of a protein of interest. Besides evaluation and optimization of process parameters (e.g. selection of the best expression host or cell line and optimal induction conditions and time points), the design of the expression construct itself has a major impact. However, the path t...