Background The envelope protein (E protein) of dengue virus is responsible for binding the virus to the host cell. This protein is considered an important immunogen for neutralization of the virus, the only able to induce the production of neutralizing antibodies [1]. The aim of this study was to use the Cowpea Mosaic Virus (CPMV) as a vector to express, on cowpea plants, Vigna unguiculata L. (...

In this work we compared, in the same immunization schedule in mice, two vaccine candidates against Dengue-4 based on the envelope protein domain III and the carrier P64k. Their molecular and antigenic features, immunogenicity and protective capacity in the viral encephalitis murine model are assessed. Our study endorses the strategy of using both sites of inclusion within the same P64k molecul...

The putative receptor-binding domain (domain III) of the flavivirus Langat envelope glycoprotein has been crystallized using the hanging-drop vapor-diffusion method at 277 K. Two distinct crystal morphologies were observed to grow under the same conditions. The crystal forms both belong to a trigonal space group, P3(1)21 or P3(2)21, with unit-cell parameters a = 80.93, c = 132.1 A and a = 104.8...

To protect against dengue viral infection, a novel lipidated dengue subunit vaccine was rationally designed to contain the consensus amino acid sequences derived from four serotypes of dengue viruses. We found that the lipidated consensus dengue virus envelope protein domain III (LcED III) is capable of activating antigen-presenting cells and enhancing cellular and humoral immune responses. A s...